| 基于线粒体 Cyt b 基因序列的洞庭湖河蚬遗传多样性分析 |
| Genetic Diversity Analysis of Corbicula fluminea in Dongting Lake Based on the Mitochondrial Cyt b Gene |
| 投稿时间：2017-08-23 修订日期：2018-07-17 |
| DOI：10.15928/j.1674-3075.2018.05.013 |
| 中文关键词:河蚬 线粒体细胞色素b 遗传多样性 遗传结构 洞庭湖 |
| 英文关键词:Corbicula fluminea Cyt b gene genetic diversity genetic structure Dongting Lake |
| 基金项目:国家自然科学基金项目 (31772832)；国家公益性行业 (农业) 科研专项 (201503108) 。 |
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| 中文摘要: |
| 分析洞庭湖不同地理群体河蚬的遗传多样性和遗传结构，为其资源合理开发和有效保护提供参考。在6个样点共采集河蚬126只，以线粒体细胞色素 b 基因部分序列为分子标记。获得长 531 bp 的序列片段，共检出 5 种单倍型，变异位点10 个；平均单倍型多样性和核苷酸多样性分别为 0.559 和 0.006。6 个河蚬群体间的遗传距离为 0.006~0.007；遗传分化指数和 AMOVA 分析显示，河蚬群体间遗传分化低，遗传变异主要来自群体个体内。最大似然法构建的系统进化树中，单倍型 DTH1 和 DTH5 聚为一支，DTH2、DTH3、DTH4 聚为另一支。中性检验结果显示洞庭湖河蚬群体相对稳定，没有经历过瓶颈效应和突然的群体萎缩等历史事件。洞庭湖 6 个河蚬群体间不存在显著的遗传差异，可将其作为一个整体进行管理和保护。 |
| 英文摘要: |
| In order to better utilize and preserve the Corbicula fluminea reserves in Dongting Lake, the genetic diversity and structure of C. fluminea from six geographical locations were examined using the partial mitochondrial cytochrome b (Cyt b) sequence (531 bp) as the genetic marker. Ten mutations and 521 conservative loci were detected in the Cyt b gene fragments from 126 C. fluminea and five haplotypes were identified (DTH1-5). The mean haplotype diversity and nucleotide diversity were 0.559 and 0.006, respectively. The genetic distance among the six C. fluminea populations ranged from 0.006 to 0.007. Analysis of the fixation index and molecular variance (AMOVA) showed that the genetic variance was primarily from variation among individuals within populations and there was no significant genetic difference among populations. In the ML phylogenetic tree, the five haplotypes were classified into two clades; the combination of DTH1 and DTH5 form one clade and DTH2-4 the other. The neutral test indicates that the C. fluminea population in the Dongting Lake is relatively stable and has not experienced events such as the bottleneck effect or sudden group atrophy. The results, taken together, show that genetic differences among the six populations are insignificant and C. fluminea in the Dongting Lake should be managed and protected as a whole. |
| 王剑平,朱鹏飞,王健,桂雨婷,李德亮.2018.基于线粒体 Cyt b 基因序列的洞庭湖河蚬遗传多样性分析[J].水生态学杂志,39(5):89-94. |
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