| 长丝裂腹鱼全人工繁殖试验 |
| Artificial Propagation of Schizothorax dolichonema |
| 投稿时间：2015-07-15 修订日期：2016-07-28 |
| DOI：10.15928/j.1674-3075.2016.04.016 |
| 中文关键词:长丝裂腹鱼 人工繁殖 试验 |
| 英文关键词:Schizothorax dolichonema, artificial propagation, enhancement and release |
| 基金项目:云南省农业科技推广项目（2011-2016） |
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| 中文摘要: |
| 对野外采捕内塘人工驯养成熟的长丝裂腹鱼开展人工繁殖试验，为长丝裂腹鱼养殖及增殖放流奠定基础。2008年在金沙江丽江境内采集到32尾50~500g长丝裂腹鱼，在大理州裂腹鱼原种场经过7年驯养。2015年4月7日催产雌鱼15尾（均重3000g）、雄鱼9尾（均重1500 g）， 亲鱼在50mg/L MS-222溶液中麻醉后注射鲤脑下垂体和绒毛膜促性腺激素混合溶液。效应时间48 h 20 min；6尾雌鱼产卵，获得鱼卵64790粒，催产率40%；受精卵23350粒，受精率36%；获得鱼苗10675尾，出苗率45.7%。 |
| 英文摘要: |
| Schizothorax dolichonema Herzenstein is a Cyprinidae fish species, endemic to the Jinsha and Yagong river basins and listed in the artificial breeding and release program for Longkaikou, Ahai and Ludila hydropower stations on the middle Jinsha River. Schizothorax dolichonema has become very rare and the need for artificial propagation, conservation and recovery is urgent. This article describes the testing of sexually mature specimens to provide a foundation for the artificial culture and release of Schizothorax dolichonema. In 2008, 28 Schizothorax dolichonema, weighing from 50 to 500 g, were collected from the Lijiang section of Jinsha River and domesticated in ponds at the Original Schizothoracinae Seed Farm of Dali Prefecture. After 7 years of domestication, a test of the complete artificial propagation was carried out in 2015. On April 7, 2015, 15 females, with an average weight of 3000g, and 9 males, with an average weight of 1500g, were induced to spawn. After being anaesthetized in a 50mg/L solution of tricaine methanesulfonate (MS-222), the fish were injected with a mixed solution of carp hypophysis and chorionic gonadotrophin at a dose of 0.5mL/kg for females and half the dose for males. After 48 hours and 20 minutes, 6 female fish spawned 64,790 eggs (spawning rate, 40%). Through artificial insemination, 23,350 fertilized eggs were obtained (fertilization rate, 36%). Incubation of zygotes was carried out at the temperature of 15 -16 ℃ and the zygotes developed to the mid-gastrula stage 40 h after insemination. On April 16 at 15:00, cleavage began and was complete the next day by 18:00. A total of 10,675 larvae hatched (incubation rate, 45.7%). The successful artificial breeding of Schizothorax dolichonema lays a solid foundation for an artificial breeding and release program for the cascade stations on the middle Jinsha River, |
| 赵树海,杨光清,宝建红,赵振寰,华朝斌,郭海涛,李根荃,董现鲜,李志洪,严燕群,苏银梅,杨弢.2016.长丝裂腹鱼全人工繁殖试验[J].水生态学杂志,37(4):101-104. |
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