| 镉胁迫对泥鳅金属硫蛋白基因表达的影响 |
| Effects of Cadmium Exposure on Metallothionein Gene Expression of Misgurnus anguillicaudatus |
| 投稿时间：2014-01-22 修订日期：2014-02-25 |
| DOI： |
| 中文关键词:泥鳅 镉胁迫 金属硫蛋白基因 |
| 英文关键词:Misgurnus anguillicaudatus cadmium exposure metallothionein gene |
| 基金项目:江苏省科技厅项目（BE2012354）、江苏省水产三项工程项目（PJ2011-62）、江苏省农业自主创新项目（CX（13）2042） |
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| 中文摘要: |
| 采用实时荧光定量PCR（Real-time PCR）分析了不同浓度Cd2+（0、0.25、2.5 mg/L）胁迫24、48、72 h对泥鳅（Misgurnus anguillicaudatus）肝脏和鳃中金属硫蛋白（MT）基因的相对表达水平的影响。结果表明，泥鳅肝脏和鳃MT基因的表达对外源Cd2+胁迫所表现出的反应存在差异，肝脏比鳃更为敏感;Cd2+浓度越高，胁迫时间越长，则肝脏和鳃中MT基因的相对表达水平越高。在受到Cd2+胁迫时，肝脏和鳃中大量表达MT，可降低Cd2+对机体造成的损伤。泥鳅MT基因可以作为监测水环境中镉污染的生物标志物之一。
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| 英文摘要: |
| This article studied the effects of different concentrations of Cd2+ (0, 0.25, 2.5mg/L) exposure on MT gene expression in liver and gill of Misgurnus anguillicaudatus for 24, 48 and 72 hours by analyzing real-time PCR data. The results indicated that there was difference in MT gene expression between the liver and gill after exposure to Cd2+, and the liver was more sensitive than gill in response to Cd2+ exposure. MT gene expression increased with the increase of Cd2+ concentration and the extension of exposure time. Under the stress of Cd2+, MT in the liver and gill will highly express to protect organism from damage. Therefore, MT gene of Misgurnus anguillicaudatus can be considered as a biomarker of Cd2+ pollution in water environment.
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| 凌去非,李彩娟,王 磊.2014.镉胁迫对泥鳅金属硫蛋白基因表达的影响[J].水生态学杂志,35(3):88-93. |
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