| 咪唑类离子液体处理对草鱼CYP3A和PXR基因表达的影响 |
| Effects of the Treatment of Imidazolium Ionic Liquids on Gene Expression of Cytochrome P450 3A and Pregrane X Receptor (PXR) in Ctenopharyngodon idellus |
| 投稿时间：2013-06-04 修订日期：2013-07-10 |
| DOI： |
| 中文关键词:离子液体 草鱼 细胞色素P450 3A 孕烷X受体 |
| 英文关键词:ionic liquids Ctenopharyngodon idellus CYP3A PXR |
| 基金项目: |
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| 摘要点击次数: 1950 |
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| 中文摘要: |
| 细胞色素Ｐ４５０是一类含有亚铁血红素的超家族单加氧酶，参与许多外源物质（包括药物、环境化合物等）和内源性物质（如激素）的代谢转化。ＣＹＰ３Ａ是Ｉ相解毒酶系Ｐ４５０家族中的重要成员，在肝脏对外源毒物解毒和内源激素的代谢过程中发挥重要作用。为探讨草鱼（Ctenopharygodon idellus）肝脏ＣＹＰ３Ａ在溴化１-辛基３-甲基咪唑离子液体（［Ｃ８ｍｉｍ］Br）的解毒过程作用以及鱼体对［Ｃ８ｍｉｍ］Br的解毒是否通过ＰＸＲ-ＣＹＰ３Ａ转录调控途径作用，通过预试验获取［Ｃ８ｍｉｍ］Br对草鱼的急性毒性效应，选取２２ｍｇ／Ｌ（１／１０ＬＣ５０）和１０９ｍｇ／Ｌ（１／２ＬＣ５０）浓度组［Ｃ８ｍｉｍ］Br对草鱼染毒２４ｈ，荧光定量ＰＣＲ检测ＣＹＰ３Ａ和ＰＸＲ的表达情况。结果表明，草鱼肝脏ＣＹＰ３Ａ水平与对照组相比极显著上升（Ｐ＜００１），ＰＸＲ基因表达量与对照组相比也极显著升高（Ｐ＜０．０１），ＣＹＰ３Ａ与ＰＸＲ基因表达水平呈现出一定的相关性；推测在草鱼体内ＰＸＲ可能就是［Ｃ８ｍｉｍ］Ｂｒ作用于ＣＹＰ３Ａ的受体。 |
| 英文摘要: |
| Cytochrome P450(CYPs ) enzymes，which was a kind of super family monooxygena with heme，joined the metabolic transformation of several exogenous materials(included medicine，environmental compounds and so on)and endogenous materials(such as hormones).CYP3A was an important member of the phrase I of detoxification enzymes P450，and it played a critical role in detoxifying exogenous toxicant and metabolizing endogenous hormone in liver .The study aimed to evaluate the function of CYP3A in the detoxification process of brominating the 1-octyl-3-methylimidazolium ionic liquid [C8mim] Br and to determine whether the detoxification of [C8mim]Br through the PXR-CYP3A transcriptional regulatory pathway or not in Ctenopharyngodon idellus. According to the results of acute toxicity tests，the [C8mim]Br-exposure concentrations were designed to be 22 mg/L and 109 mg/L for Ctenopharyngodon idellus for 24 hours，and the expression situation of CYR3A and PXR was detected by fluorescent quantitative PCR. The result indicated that [C8mim]Br significantly up-regulate the expression of CYP3Aand PXR at mRNA level in fish liver( P<0. O1)，and the gene expression level of CYP 3A and PXR presented a certain correlation. This result assumed that the PXR in Ctenopharyngodon idellu.s maybe the receptor of [C8mim] Br on CYP3A.
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| 马军国,周春娥,李效宇.2013.咪唑类离子液体处理对草鱼CYP3A和PXR基因表达的影响[J].水生态学杂志,34(6):70-75. |
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