西藏裂腹鱼类出血病的病原分析
Etiological analysis of Hemorrhagic disease in Tibetan Schizothorax
投稿时间:2012-11-07  修订日期:2012-12-31
DOI:
中文关键词:裂腹鱼类  出血病  病原  西藏  
英文关键词:Schizothorax  hemorrhagic disease  pathogen  Tibet
基金项目:
作者单位E-mail
强巴央宗* 西藏农牧学院动物科学学院西藏林芝 860000 wangjiu28@126.com 
旦增旺久 西藏农牧学院动物科学学院  
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中文摘要:
       拉萨裸裂尻鱼(Schizopygopsis younghusbandi Regan)、拉萨裂腹鱼(Schizothorax waltoni Regan)和异齿裂腹鱼(Schizothorax o’connori Lloyd)来源于雅鲁藏布江米林江段,采集自西藏林芝地区八一镇农贸市场,规格250~300g。从裂腹鱼病鱼的肌肉、肠道、肝脏、肾脏等组织中共分离到12种细菌。用病鱼匀浆组织的超微(0.2μm)过滤悬液和12种细菌悬液感染健康的裂腹鱼,发现超微过滤悬液未导致出血病,3种细菌悬液具有较强的致病力,病鱼有典型的出血病症状,死亡率87%、100%和100%。革兰氏染色后镜检观察,该3种菌株均为革兰氏阴性杆菌,少数成对或短链状,菌种未鉴定。   
英文摘要:
       This experiment was conducted to identify the etiology of hemorrhagic disease in three species of Schizothtorax (Schizopygopsis younghusbandi, Schizothorax waltoni, and Schizothorax o’connori) peculiar to Tibet. Fishes with hemorrhagic disease were obtained from local market for pathogens isolation and artificial infection. 12 pathogenic bacteria were isolated from muscle, intestine, liver and kidney of the three species. Through infection of health Tibetan Schizothorax with virus suspension of diseased tissues and the 12 isolated bacteria, it was found that virus was not the pathogen of hemorrhagic disease, and 3 pathogenic bacteria showed strong pathogenicity. The results suggested that the hemorrhagic disease was caused by bacterial pathogen in three species of Schizothoracinae fishes. This can provide scientific evidence and theoretical basis to protection and exploitation of endemic fishes of Tibet.
强巴央宗,旦增旺久.2012.西藏裂腹鱼类出血病的病原分析[J].水生态学杂志,33(6):124-127.
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