| 不同规格黄鳝消化酶活性的比较 |
| Comparison of Digestive Enzyme Activities in Monopterus albus of Different Sizes |
| 投稿时间：2012-02-22 修订日期：2012-09-21 |
| DOI： |
| 中文关键词:黄鳝 规格 淀粉酶 蛋白酶 脂肪酶 |
| 英文关键词:Monopterus albus sizes protease amylase lipase |
| 基金项目: 国家星火计划重大专项(2011GA770007)； 公益性行业专项(201003076);湖南省科技重大专项(2010FJ1007);湖南农业大学人才引进项目(08YJ01) |
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| 中文摘要: |
| 以相同环境条件下饲养2个月的黄鳝(Monopterus albus)为试验对象,以酶学方法研究3种规格[(143.81±36.27)g、(76.63±14.43)g、(31.35±5.82)g]黄鳝的肝脏、前肠、后肠蛋白酶、脂肪酶和淀粉酶的活性以及消化酶(蛋白酶、脂肪酶和淀粉酶)在肝脏、前肠、后肠中的活性分布。结果表明,黄鳝蛋白酶活性从高到低依次为前肠>后肠>肝脏;淀粉酶活性从高到低依次为后肠>前肠>肝脏;脂肪酶活性从高到低依次为后肠>前肠>肝脏;随着黄鳝体重的增加,肠道蛋白酶活性逐渐升高,而肝脏蛋白酶活性变化不明显;后肠、肝脏淀粉酶活性随着黄鳝体重的增加而升高,而前肠淀粉酶活性降低;黄鳝脂肪酶活性有随着规格变大先升高、后降低的趋势,以中规格黄鳝消化组织的脂肪酶活性最高。 |
| 英文摘要: |
| Monopterus albus of three sizes in this study were cultured for two mouths in the same aquaculture condition. The activities and distributions of protease，lipase and amylase in liver，fore-intestine and hind-intestine of M. albus of three sizes[(143.81± 36. 27)g，(76. 63 ±14. 43)g，and(31.35 ±5.82 g )〕were examined by using enzyme analytical methods. Results obtained here showed that : the decreasing order of protease activity in M. albus was fore-intestine>hind-intestine>liver, whilst the decreasing order of amylase and lipase activity in M. albus was hind-intestine>fore-intestine>liver ;as body weight of M. albus increased，intestinal protease activity gradually increased and the protease activity in liver did not change significantly ;the amylase activities in hind-intestine and liver increased with the body weight increasing，however, the amylase activity in fore-intestine had an opposite trend; M. albus in medium一size presented higher lipase activity than those in big一size and small-size. |
| 郇志利,何蓝波,胡毅,戴振炎,王晓清,肖调义,夏 敏,唐华钟.2012.不同规格黄鳝消化酶活性的比较[J].水生态学杂志,33(5):57-60. |
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