| Hg2+胁迫对三种微藻生长和叶绿素荧光特性的影响 |
| Effects of Hg2+ Exposure on the Growth and Chlorophyll Fluorescence of Three Microalgal Strains |
| 投稿时间：2011-08-29 修订日期：2011-10-12 |
| DOI： |
| 中文关键词:微藻 Hg2+ 叶绿素荧光 细胞密度 |
| 英文关键词:microalgae Hg2+ chlorophyll fluorescence cell density |
| 基金项目:水利部948项目“藻类在线水体生态毒性监测系统”(合同号:200902);环境保护部水体污染控制与治理科技重大专项(2009ZX07528-003) |
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| 中文摘要: |
| 研究了不同浓度的Hg2+胁迫不同时间(1～7 d)后,蛋白核小球藻(Chlorella pyrenoidosa)、斜生栅藻(Scenedesmus obliquus)和莱茵衣藻(Chlamydomonas reinhardtii)生长及叶绿素荧光特性的变化情况。测定的主要参数有光系统II(PSII)的最大光能转化效率(ΦM)、相对光合电子传递效率(rETR)、饱和光强(Ik)和细胞密度。试验结果表明,Hg2+胁迫下3种微藻的细胞密度和叶绿素荧光参数与Hg2+浓度、胁迫时间有一定的相关关系,细胞密度和叶绿素荧光各参数间有极显著的相关关系(P<0.01)。通过计算Hg2+对3种微藻不同胁迫时间的半抑制浓度(EC50),发现3种微藻对Hg2+的耐受性大小顺序为:斜生栅藻>莱茵衣藻>蛋白核小球藻。 |
| 英文摘要: |
| The present study investigated the growth and chlorophyll fluorescence characteristics of 3 microalgal strains(Chlorella pyrenoidosa，Scenerlesmus obliquus and Chlamydomonas reinhardtii ) under different Hgz+ concentrations and different durations(1一7 d).The main parameters including the maximal photochemical efficiency of PSII ( ФM)，the relative electron transport rate (rETR )，the saturation intensity(Ik)and cell density were determined. The results showed that the chlorophyll fluorescence and cell density of 3 microalgal strains all exhibit some correlations to Hg2+ concentration and exposure duration. While cell density has very significant positive correlations(P<0.01)to fluorescence parameters. Comparison of the EC50 values of different exposure durations among the 3 algal species indicated the ability of their tolerance to Hg2+ was S. obliquus>C. reinhardtii>C. pyrenoidosa. |
| 李明,潘晓洁,邹怡,陈晓娟,万成炎.2012.Hg2+胁迫对三种微藻生长和叶绿素荧光特性的影响[J].水生态学杂志,33(2):96-99. |
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