赣江中游四大家鱼遗传多样性的ISSR分析
Study of Four Major Chinese Carps with Inter Simple Sequence Repeat( ISSR)Markers in the Middle Reach of Ganjiang River
投稿时间:2011-02-10  修订日期:2011-02-10
DOI:
中文关键词:赣江中游  四大家鱼  ISSR  
英文关键词:Middle reach of Ganjiang River  Four major Chinese carps  ISSR
基金项目:国家自然科学基金(30760188)资助
作者单位E-mail
张建铭* 江西省赣州市水产研究所江西赣州 341000 blueidea@163.com 
吴志强 桂林理工大学环境科学与工程学院广西桂林 541001  
胡茂林 南昌大学生命科学与食品工程学院江西南昌 330031  
曾庆祥 江西省赣州市水产研究所江西赣州 341000  
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中文摘要:
      利用ISSR分子标记技术对赣江中游四大家鱼的遗传多样性进行分析,青鱼(Mylopharyngodon piceus)、草鱼(Ctenopharyngodon idellus)、鲢(Hypophthalmichthys molitrix)、鳙(Aristichthys nobilis)分别扩增出47、74、55、59个位点,其中多态性位点数分别为33、50、29、30,多态性位点比例分别为70.21%、67.57%、52.73%、51.72%。青鱼的Nei’s基因多样性和Shannon信息指数分别为0.2606和0.3846,草鱼为0.2391和0.3560,鲢为0.1858和0.2769,鳙为0.1908和0.2827。结果显示,赣江四大家鱼群体均存在一定的遗传变异空间,其遗传多样性相对较丰富。
英文摘要:
       The colonial genetic variations in four major Chinese carps were analyzed with ISSR,which generated 47(black carp ),74(grass carp),55(silver carp ) and 59(bighead carp) bands. The number of polymorphic band were 33,50,29,30,and the percentage of polmoryphism were 70. 21%,67. 57%,52. 73 %,51.72 %.The index of Nei's gene diversity and Genetic diversity quantified by Shannon Index varied of black carp were 0. 2606 and0.3846,grass carp were 0. 2391 and 0. 3560,silver carp were 0.1858 and 0.2769,bighead carp were 0.1908 and 0.2827. The experiment results showed the heredity and variation space was a little larger in the four major Chinese carps in Xiajiang reach of Ganjiang River, their colonial genetic diversity were abundant.
张建铭,吴志强,胡茂林,曾庆祥.2011.赣江中游四大家鱼遗传多样性的ISSR分析[J].水生态学杂志,32(5):69-73.
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