| 转基因与野生唐鱼耐温限度及窒息点的比较研究 |
| Comparative Study on Extreme Temperature Tolerance and Asphyxiation Point of Red Fluorescent Protein Transgenic and Wild-type Tanichthys albonubes |
| 投稿时间：2010-03-31 修订日期：2010-11-03 |
| DOI： |
| 中文关键词:唐鱼 转基因 温度 窒息点 |
| 英文关键词:Tanichthys albonubes Transgenic Temperature Asphyxiation point |
| 基金项目:国家科技基础条件平台工作项目（Ｎｏ２００５ＤＫＡ２１１０３）；广东省海洋渔业科技推广专项项目（Ｎｏ.Ａ２００８９９Ｆ０１）和广东省科技计划项目（Ｎｏ．２００７Ｂ０２０７０８００８）资助。 |
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| 中文摘要: |
| 对转红色荧光蛋白基因唐鱼、野生型唐鱼Ⅰ和野生型唐鱼Ⅱ的耐温和耐氧能力进行比较研究。结果表明，在驯化温度为３０℃，升温速率为１℃／ｈ时，３种唐鱼的半致死高温分别为
（３８.５３±０.０８）℃、（３８.２８±０.２９）℃和（３８.５５±０.０９）℃。在驯化温度为２５℃，降温速率为２℃／ｄ时，３种唐鱼的半致死低温分别为（１.１３±
０．40）℃、（１.２６±０.１０）℃和（１.２１±０.１２）℃；恒温２５℃时，测定３种唐鱼的窒息点分别为（０．６６±０．０２）ｍｇ／Ｌ、（０．７４±０.２１）ｍｇ／Ｌ和
（０.５１±０.０１）ｍｇ／Ｌ。差异显著性检验表明，３种唐鱼对温度和溶氧的耐受力无显著性差异（Ｐ＞０.０５）。 |
| 英文摘要: |
| The tolerance to extreme water temperature and lower dissolved oxygen were compared among the red fluorescent protein transgenic Tanichthys albonubes，wild-type fish I and wild-type fish .The results were as follows: when the acclimation temperature was 30℃，and the rate of temperature increase was approximately 1℃/h，the high semi-lethal temperatures of three different stains were(38. 53 ±0. 08) ℃，(38. 28 ±0. 29)and(38. 55± 0. 09) ℃，respectively. When the acclimation temperature was 25℃，and the rate of temperature decrease was 2℃ /d，the low semi-lethal temperatures of three different stains were(1 .13±0. 40) ℃，(1.26 ±0.10 ) ℃ and(1 .21 ±0. 12) ℃，respectively. Under constant temperature 25℃，the asphyxiation points of them were (0. 66 ±0. 02)mg/L,(0. 74±0. 21)mg/L and (0.51±0.01)mg/L, respectively. Significance test of difference indicated that there were no significant differences (P<0.05) in the tolerance to extreme water temperature and dissolved oxygen among three strains of T. albonubes .
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| 姜鹏,白俊杰,樊佳佳.2010.转基因与野生唐鱼耐温限度及窒息点的比较研究[J].水生态学杂志,31(6):92-95. |
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