| 斑点叉尾鮰病原细菌的分离鉴定及系统发育分析 |
| Isolation, identification and phylogenetic analysis of pathogenic bacteria in Ictalurus punctatu |
| 投稿时间：2010-03-20 修订日期：2010-04-25 |
| DOI： |
| 中文关键词:斑点叉尾鮰 病原细菌 16S rDNA 系统发育分析 |
| 英文关键词:Ictalurus Punctatus pathogenic bacteria 16S rDNA phylogenetic analysis |
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| 中文摘要: |
| 用传统的微生物学分离培养方法，从斑点叉尾!（Ｉｃｔａｌｕｒｕｓｐｕｎｃｔａｔｕｓ）的鳃、肠和肝等器官共分离到１６株可疑病原细菌（编号为ＷＪ０１～１６），以提取的基因组ＤＮＡ为模版，扩增出约６００ｂｐ大小的１６ＳｒＤＮＡ片段，测序并与ＧｅｎＢａｎｋ数据库中的已知序列进行比对。１６株细菌中，９株同源性在９８％以上，５株在９５％以上，２株为９２％。系统发育分析表明，１６株细菌明显地聚为３支。 |
| 英文摘要: |
| Sixteen strains of suspicious pathogenic bacteria were isolated from the branchias，intestines and livers of punctatus with the tranditional microbiology method. Used the isolated genome DNA as 600bp fragments of 16S rDNA of sixteen strains were amplified by PCR method. The sequence analysis indicated that the similarity value of nine strains of the sixteen bacteria are 98%一100 %, five are 95%-97 % and two are 92%，by comparing the 16S rDNA sequence of the isolated strains whth the relative bacteria in the Gen-Bank database. The phylogenetic analysis showed that the sixteen strains of bacteria obviously assembled three branches.
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| 宋培勇,王庆容,李 顺,杨秀荣.2010.斑点叉尾鮰病原细菌的分离鉴定及系统发育分析[J].水生态学杂志,31(5):62-65. |
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