| 低浓度铜暴露导致梨形环棱螺氧化胁迫及DNA损伤的研究 |
| Exposure to trace copper in Bellamya purificata inducing DNA damage and oxidative stress |
| 投稿时间：2010-03-15 修订日期：2010-05-24 |
| DOI： |
| 中文关键词:Cu2+ DNA损伤 氧化胁迫 梨形环棱螺 |
| 英文关键词:Cu2+ DNA damage Oxidative stress Bellumya purificata |
| 基金项目:国家自然科学基金资助项目（Ｎｏ．３０４７０３４２）；中国水产科学研究院淡水生态与健康养殖重点开放实验室项目（Ｎｏ．００７ＦＥＡ０２０５）；中国水产科学研究院内陆渔业生态环境和资源重点开放实验室项目（Ｎｏ．ＹＭ２００７－０３）；湖北师范学院２００８年度人才项目（Ｎｏ．２００８Ｆ１４）及湖北省教育厅２０１０年度重点项目资助。 |
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| 中文摘要: |
| 采用暴露重金属的方法，研究了不同浓度硫酸铜（Ｃｕ２＋分别为０００、０．００５、０．０１、０．０２、０．０５ｍｇ／Ｌ）在不同暴露时间（０～１４ｄ）下对梨形环棱螺（Ｂｅｌｌａｍｙａｐｕｒｉｆｉｃａｔａ）肝脏和鳃组织线粒体的活性氧（ＲＯＳ）含量、肝脏和鳃组织中金属硫蛋白（ＭＴ）含量和ＤＮＡ单链断裂程度的影响，探讨Ｃｕ２＋对梨形环棱螺的氧化胁迫和ＤＮＡ损伤及ＭＴ防御作用的机理。结果表明，Ｃｕ２＋对梨形环棱螺肝脏和鳃线粒体ＲＯＳ水平、肝脏和鳃组织ＤＮＡ损伤和ＭＴ含量均有明显的影响。ＲＯＳ含量在实验开始时持续上升，肝脏和鳃线粒体ＲＯＳ分别在第７天（除０．００５ｍｇ／Ｌ剂量组外）和第４天达到最高点，随后下降，但直到第１０天时仍显著高于对照组。肝脏和鳃组织ＤＮＡ单链断裂程度随Ｃｕ２＋暴露剂量和暴露时间而显著增加。暴露于Ｃｕ２＋后，Ｆ值迅速降低，随后，Ｆ值开始上升，在第２天时的Ｆ值达到峰值；随后ＤＮＡ单链断裂程度继续加重，第５天后Ｆ值持续下降，至第１０～１４天时达到稳定水平。第１０～１４天时，肝脏组织０．００５、０．０１、０．０２和０．０５ｍｇ／Ｌ剂量组间的Ｆ值没有显著差异。ＭＴ在整个实验期间均处于诱
导状态，０．０１ｍｇ／Ｌ剂量组肝脏的ＭＴ在整个实验期间均被极显著地诱导（Ｐ＜０．０１），０．０１ｍｇ／Ｌ剂量组的鳃组织ＭＴ除第１０天外也被极显著诱导（Ｐ＜０．０１）。在暴露至第１４天时，除０．０５ｍｇ／Ｌ剂量组的肝脏ＭＴ外，其余均处于极显著诱导状态（Ｐ＜０．０１）。
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| 英文摘要: |
| An exposure experiment was carried out to study the effects of copper at different concentrations(Cu2+: 0.00，0. 005，0.01，0. 02，0.05 mg/L ) and different time (0-14 d) on concentrations of reactive oxygen species(ROS ) and metallothionein(MT) and DNA single-strand breaking in livers and gills of Bellamya purificat，in order to evaluate the mechanisms of oxidative stress，DNA damnification and recovery of MT in B. purificat，which are induced by Cu2+. Gills and livers were chosen to evaluate these toxicological responses because they are the organs which are direct contact with environmental pollutants. The results showed that Cu2+ had significant influence on concentrations of ROS and MT，DNA single-strand breaking in gills and livers of B. purificat. The concentrations of ROS keeped increasing in the beginning of exposure，and they reached a peak value in livers on the 7th day(except the treatments with 0. 005 mg/L) and in gills on the 4th day，and then the concentrations of ROS decreased，but they were still significantly higher than that of control group on the first 10 day. The extents of DNA single-strand breaking in livers and gills of B. purificata was significantly increased comparing with the control group. On the first day during exposure to Cu2+，the F value decreased quickly，and then increased and reached a peak value till on the second day of exposure. Subsequently，the extents of DNA single-strand breaking deepened and the F value keep decreasing clearly. During the 10th to 14th days，the F values of DNA single-strand breaking maintained at a steady level，and there weren't significant difference among the groups of 0. 005，0. 01，0. 02 and 0.05mg/L vs control groups in livers of B. purificata. The concentration of metallothionein (MT) in livers and gills of B. purificata revealed inducing effect during the whole exposing process. MTs of liver and gill intreatment with 0.01 mg/L of Cu2+ was extremely significantly induced(P<0.01) except that of gill on the 10th day. On the 14th day of exposure，liver and gill MTs in all groups showed an extremely significant inducement(P<0.01)except liver MT in treatment with 0.05 mg/L of Cu2+.
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| 张清顺,熊邦熹,侯建军.2010.低浓度铜暴露导致梨形环棱螺氧化胁迫及DNA损伤的研究[J].水生态学杂志,31(5):49-55. |
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