| 刀鲚精子超微结构研究 |
| Study on the Ultrastructure of Coilia ectenes Spermatozoon |
| 投稿时间：2010-02-19 修订日期：2010-04-29 |
| DOI： |
| 中文关键词:刀鲚 精子 超微结构 |
| 英文关键词:Coilia ectenes Spermatozoon Ultrastructure |
| 基金项目:安徽省科学技术厅项目（０７０３０５０３０４９）。 |
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| 中文摘要: |
| 在光学显微镜及透射电子显微镜下观察刀鲚精子超微结构，并对精子各部分长度进行测定。研究表明，刀鲚精子由头部、中段和尾部（鞭毛）组成。头部在光学显微镜下近梭形，透射电镜下纵切则近圆形。头部无顶体，由细胞核组成，核内染色质致密，空隙少，几乎无细胞质。头部后端偏一侧处有一植入窝，内有中心粒复合体。精子中段位于核后端，由中心粒复合体和袖套组成。中心粒复合体由近端中心粒和远端中心粒组成，两者基本位于一直线上。袖套肥厚的一侧有较多的线粒体和囊泡。尾部分为主段和末段，无侧鳍。主段具典型的“９＋２”的轴丝结构，末段很短，无典型轴丝结构。通过光学显微镜测定，精子头部为（２．３４±０．１６）μｍ，中段（１．４９±０．１８）μｍ，头宽（１．２９±０．２１）μｍ，尾部长（３４．０７±４．３１）μｍ，全长（３７．７７±４．２１）μｍ。 |
| 英文摘要: |
| Ultrastructure of Coilia ectenes spermatozoon was studied with light microscope (LM ) and transmission electron microscope ( TEM)，and the lengths of different parts of spermatozoon were measured under LM. The results showed that the spermatozoon of Coilia ectenes could be mainly divided into three parts: head，midpiece and tail(flagellum). Head of spermatozoon was spindle-like shaped under LM and spherical shaped under TEM when longi-tudinally sectioned. Head was mainly composed of the nucleus，and without acrosome，and only little cytoplasma was existed .The chromatin in nucleus was dense with few less dense areas. At the caudal-lateral end of the head，there was a shallow implantation fossa，which held the centriolar complex. The midpiece was at the posterior end of the nucleus，consisted of centriolar complex and sleeve. The centriolar complex consisted of proximal centriole (PC)and distal centriole( DC). The PC and DC were in the same line. The sleeve was asymmetric. The bigger and fatter side of the sleeve had more mitochondria and veses. The tail could be divided into two parts(principal piece and end piece)，and had no lateral fins. The principal piece of the spermatozoon tail was a conventional "9+2" structure，the end piece was very short and had lost this conventional structure. The light microscopy measuring showed that head length，midpiece length，head width，tail length and total length were about 2. 34 ±0. 16 μm，1 .49 ±0. 18μm，1.29 ± 0. 21μm，34. 07 ±4. 31μm，37. 77 ± 4. 21μm respectively.
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| 王冰,万 全,李 飞,庄永龙,沈保平.2010.刀鲚精子超微结构研究[J].水生态学杂志,31(3):57-63. |
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