| 灿烂弧菌和创伤弧菌的不同保存方法比较 |
| Comparison of different preservation methods for Vibrio splendidus and Vibrio vulnificus |
| 投稿时间：2009-12-19 修订日期：2010-02-12 |
| DOI： |
| 中文关键词:灿烂弧菌 创伤弧菌 穿刺石蜡法 甘油冷冻法 |
| 英文关键词:Vibrio splendidus Vibrio vulnificus Puncture-paraffin method Glycerol cry-preservation method |
| 基金项目:浙江省科技厅科研院所专项公益技术攻关项目（2007F10009）；浙江省科技厅科研院所青年人才计划项目（2006R20001）；温州市科技兴海项目（S20070051）联合资助 |
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| 中文摘要: |
| 比较了琼脂斜面法、穿刺石蜡法和甘油冷冻法保存灿烂弧菌（Ｖｉｂｒｉｏｓｐｌｅｎｄｉｄｕｓ）和创伤弧菌（Ｖｉｂｒｉｏｖｕｌｎｉｆｉ ｃｕｓ）的效果。结果表明，甘油冷冻法和穿刺石蜡法的保存期显著比琼脂斜面法长，保存１２月后，各保存方法细菌的毒力都有不同程度的下降，甘油冷冻法显著好于其它２种保存方法。 |
| 英文摘要: |
| Three preservation methods(serially passage nn bevel method，puncture-paraffin method，glycerol cry-preservation method ) for same strain of Vibrio splendidus and Vibrio vulnificus were compared in this paper. The result showed that the preserved stage of glycerol cry-preservation method and puncture-paraffin method was longer than anther method; the virulence of Listonella anguillarum preserving twelve months decreased in different degree for three preservation methods，glycerol cry-preservation method was better than the others.
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| 闫茂仓,陈少波,单乐州,王雪鹏,马爱敏,谢起浪.2010.灿烂弧菌和创伤弧菌的不同保存方法比较[J].水生态学杂志,31(5):136-140. |
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