| 池蝶蚌mtDNA COⅠ基因序列分析 |
| Sequence Analysis of Mitochondrial COⅠ of Hyriopsis schlegeli |
| 投稿时间：2009-12-11 修订日期：2009-12-13 |
| DOI： |
| 中文关键词:池蝶蚌 线粒体 ＣＯⅠ基因 人工选育 序列分析 |
| 英文关键词:Hyriopsis schlegeli Mitochondrion CO I gene Selected breeding Sequence analysis |
| 基金项目:农业部948项目（2004－T9），国家发改办（[2005]1899） |
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| 中文摘要: |
| 以三角帆蚌（Ｈｙｒｉｏｐｓｉｓｃｕｍｉｎｇｉｉ）线粒体ＤＮＡ设计特异性引物，对池蝶蚌（Ｈ．ｓｃｈｌｅｇｅｌｉｉ）的线粒体ＣＯⅠ基因进行ＰＣＲ扩增，以期了解池蝶蚌的选育效果。结果表明：在１０个个体中均得到序列一致的ＣＯⅠ序列，长度为１５４２ｂｐ；Ａ、Ｔ、Ｇ、Ｃ含量分别为１８．１％ （２８０个）、４１．８％ （６４４个）、２５．４％ （３９２个）和１４．７％ （２２６个），Ａ＋Ｔ含量（５９．９％）明显高于Ｇ＋Ｃ含量（４０．１％），与其他无脊柱动物相同基因片段碱基含量相似。该基因中密码子Ｔ（Ｕ）的使用频率很高，第１位核苷酸中Ｔ和Ｇ的含量较为一致分别为３２．０％和３０．８％；密码子第２位上碱基Ｔ的使用比率高达４２．７％，碱基Ｇ的使用比率低至１７２％；密码子第３位上碱基Ｔ的使用比率高达５０．７％，而碱基Ｃ的使用比率仅为６６％。ＢＬＡＳＴ结果显示池蝶蚌与其他淡水贝类的ＣＯⅠ基因具有较高的同源性，其中与三角帆蚌的相似性为９５％，ＭＰ法构建的分子系统进化树表明：池蝶蚌ＣＯⅠ基因与三角帆蚌ＣＯⅠ基因亲缘关系最近。以上结果表明：池蝶蚌经过人工选育后已初步成为一个纯系，遗传性状趋于稳定。 |
| 英文摘要: |
| With the expectation to investigate the breeding effect of Hyriopsis schlegeli，mitochondrial DNA CO I gene was amplified by PCR and sequenced by two primers which were designed according to the complete mtDNA of Hyriopsis cumingi. The results showed that there was no difference in sequences among the ten samples. The number of its whole gene sequence was 1542 bp in length which had 18. 1%(280), 41.8%( 644), 25.4%(392)，14.7% (226) of A，T，G，and C，respectively，A+T(59.9%)>G+C(40. 1%)，similar to the sequences in other invertebrate animals. It had high content of T in cidons，the first positions of colons had 32. 0% content of T and 32. 8% content of G; the second positions of the colons in the gene have high content of T(42.7)but low content of G(17. 2%); 50.7% content of T and 6. 6% content of C were found in the third positions. The CO I gene of the H. Schelegii was proved to have good homology with other species of freshwater mussel recorded in the GenBank，the sequence similarity between H. Schelegii and H. cumingi. was 95%. The result of Phylogenetic tree which was constructed by Maximum Parsimony method indicated that there was the closest phylogenetic relationship between H. Schelegii and H. cumingi. It indicated that the genetic characteristic of the H. schlegeli tends to be stable and become pure strain after 3 generation artificial selected.
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| 王军花,曾柳根,林巧惠,盛军庆,徐毛喜,龚贵如,洪一江.2010.池蝶蚌mtDNA COⅠ基因序列分析[J].水生态学杂志,31(5):21-26. |
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