| 泥蚶血清及血细胞免疫功能研究 |
| Haemocytes and haemolymph immune function research on Tegillarca granosa |
| 投稿时间：2009-10-26 修订日期：2010-04-13 |
| DOI： |
| 中文关键词:泥蚶 血清 免疫功能 超氧化物歧化酶 |
| 英文关键词:Tegillarca granosa Haemocytes Immune function Superoxide dismutase |
| 基金项目:福建省教育厅A类科技项目（JA09213）；泉州师范学院大学生科研基金项目（2009DA023）；泉州师范学院自选科研项目（项目编号：2008KJ10） |
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| 中文摘要: |
| 初步研究了泥蚶（Ｔｅｇｉｌｌａｒｃａｇｒａｎｏｓａ）血细胞离体条件下的吞噬作用以及血清因子对其的影响，并测定经大肠杆菌注射以后，泥蚶血清中抗菌、溶菌、酚氧化酶（ＰＯ）、超氧化物歧化酶（ＳＯＤ）活力的变化。结果表明，泥蚶血清能促进血细胞对大肠杆菌的吞噬，但不能识别鸡红细胞。大肠杆菌激发后泥蚶血清中抗菌活力在１ｈ、１５ｈ和３０ｈ时实验组均显著高于对照组。溶菌活力实验组均高于对照组，但在１５ｈ差异不显著。ＰＯ活力实验组均低于对照组，在３０ｈ时差异极显著。ＳＯＤ活力在１ｈ时显著低于对照组，而在３０ｈ时显著高于对照组。 |
| 英文摘要: |
| The phagocytosis test on haemocytes of Tegillarca granosa vitro and the effects of haemolymph factors were preliminary studied，and the activities of antibacterial , bacteriolytic、phenoloxidase and superoxide dismutase in the haemolymph of T. granosa after immune with Escherichia coli by the way of muscular injection were also studied. The results showed that haemolymph of T. granosa can promote phagocytosis of E. coli cells, but haemocytes do not recognize chicken red blood cells. The antibacterial activity of experimental groups in serum were significantly higher than that of control groups in 1，15 and 30 h. The bacteriolytic activity of experimental groups in serum were higher than that of control groups，but 15 h was not significant difference. The PO activity in experimental group were lower than the control group，difference was very significant in the 30h. The SOD activity was significantly lower than that of control group in 1 h, but significantly higher in 15 and 30h.
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| 柯佳颖,陈寅山,戴聪杰.2010.泥蚶血清及血细胞免疫功能研究[J].水生态学杂志,31(3):80-84. |
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