| 灿烂弧菌灭活菌苗对美国红鱼免疫效果的研究 |
| Study on Immune Efficacy Induced by Killed Vibrio splendidus Vaccine on Sciaenops ocellatus |
| 投稿时间：2009-09-01 修订日期：2009-11-12 |
| DOI： |
| 中文关键词:美国红鱼，灿烂弧菌，菌苗 |
| 英文关键词:Sciaenops ocellatus Vibrio splendidus vaccine |
| 基金项目:浙江省科技厅科研院所青年人才计划项目（２００６Ｒ２０００１）；浙江省科技厅科研院所专项公益技术攻关项目（２００７Ｆ１０００９）；温州市科技兴海项目（Ｓ２００７００５１）联合资助。 |
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| 中文摘要: |
| 制备灿烂弧菌(Vibrio splendidus)福尔马林灭活菌苗,通过浸泡、注射和口服3种方式接种美国红鱼(Sci-aenops ocellatus),分别在接种免疫后第7、14、21、28、35天采鱼血,检测其血清抗体效价、溶菌酶活力和白细胞吞噬活性;第28天用1.0×108CFU/mL的灿烂弧菌悬液进行攻毒试验,检测菌苗的免疫效果。结果表明,各免疫组血清抗体效价在第21天达到峰值,且浸泡组和注射组的血清抗体效价高于口服组;在35d实验期内,各免疫组的血清溶菌酶活力有明显提高,各浸泡组和注射组于免疫接种后14d显著高于对照组(P<0.05);除浸泡3组外,其余各免疫组的白细胞吞噬百分比(PP)和吞噬指数(PI)在免疫接种后14d均较对照组有显著差异(P<0.05);各组受免鱼对人工攻毒均具有保护作用,以注射2组的免疫保护率最高,达77.8%。 |
| 英文摘要: |
| Sciaenops ocellatus were vaccinated with a formalin-killed Vibrio splendidus vaccine via immersion，injection and oral route，respectively. Antibody titers，lysozymes，leucocyte phagocytosis were examined on the 7th，14th，21st，28th d after first vaccination. A dosage of 0.2 mL of V. splendidus 1.0 x 108CFU/mL was intraperitoneally injected to the vaccinated fish to evaluate the protective efficiency of the vaccine. The results indicated that: the antibody titers of all immunized group was the highest on the 21st d and that of all immersion group and injection group were higher than all oral route group; lysozyme of all immunization groups increased，and all immersion group and injection group showed significant difference compared with controlled group ( P<0.05)after the 14th-vaccination; The phagocytic percentage(PP) and phagocytic index(PI) of all immunized groups except the immersion group 3 were showed significant difference compared with controlled group(P<0.05)after the 14th-accination，14 d after challenge with V. splendidus，the relative percentage survival(RPS ) values showed that the vaccination of injection group 2 was the best of all.
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| 马爱敏,闫茂仓,胡利华,谢起浪,陈少波,单乐州.2010.灿烂弧菌灭活菌苗对美国红鱼免疫效果的研究[J].水生态学杂志,31(3):137-141. |
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