| 锌锰组合对盐藻生长和蛋白质、β－胡萝卜素积累的影响 |
| Effects on Growth and Accumulation of Protein and βcarotene in Dunaliella salina Influenced by Combination of Zn and Mn |
| 投稿时间：2009-08-21 修订日期：2010-05-11 |
| DOI： |
| 中文关键词:锌锰组合，盐藻，物质积累 |
| 英文关键词:Combination of Zn and Mn Dunaliella salina Growth Material accumulation |
| 基金项目:江苏省海洋生物技术重点实验室开放课题项目（ＨＳ０８０１０），淮海工学院自然科学基金（Ｚ２００７０３３）。 |
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| 中文摘要: |
| 为了探索培养盐藻的微量元素条件，实验研究了微量元素锌、锰的不同浓度组合对盐藻细胞生长与物质积累的影响。结果表明，培养液中适当锌锰浓度的组合对盐藻细胞生长和物质积累有促进作用，锌锰浓度过高或过低都是相对不利的。在试验的９种锌锰浓度组合中，以培养液中
ｎＳＯ４·７Ｈ２Ｏ６ｍｇ／Ｌ和ＭｎＣｌ２·４Ｈ２Ｏ４ｍｇ／Ｌ的浓度组合对盐藻细胞的生长和物质积累促进作用最好，它可使培养液中的盐藻细胞密度、蛋白质积累量和β 胡萝卜素积累量都达到最高。培养液中ＺｎＳＯ４·７Ｈ２Ｏ８ｍｇ／Ｌ和ＭｎＣｌ２·４Ｈ２Ｏ６ｍｇ／Ｌ的浓度组合可使单个盐藻细胞中β 胡萝卜素和蛋白质积累量都最高，但可能仅是盐藻细胞在锌、锰过多的逆境条件下的适应性反应。 |
| 英文摘要: |
| In order to research the microelement concentration to Dunaliella salina cell cultured，the experiment study on that effects on growth and material accumulation of Dunaliella salina influenced by different concentration combination of Zn and Mn. The results showed that adequate concentration of Zn and Mn Cl2•4H2O had improving to Dunaliella salina cell growth and material accumulation，it was disadvantageous when concentration was too high or too low. when the combination was in a ZnSO4•7 H2O concentration of 6 mg/L and Mn concentration of 4 mg/L in culture medium，the effects were most advantageous to growth，accumulation of protein and β-carotene in Dunaliella salina in 9 different patterns. Made cellular density，protein andβ-carotene accumulation of Dunaliella salina to reach high limit among. When the combination was in a ZnSO4•7H2O concentration of 8 mg/L and MnCl2•4H2O concentration of 6 mg/L，they made protein andβ-carotene accumulation in single cell of Dunaliella salina to reach high limit among. The latter may be a response adaptation of Dunaliella salina cell to adversity of too much Zn and Mn.
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| 郭金耀,杨晓玲.2010.锌锰组合对盐藻生长和蛋白质、β－胡萝卜素积累的影响[J].水生态学杂志,31(4):127-130. |
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