| 中国沿海5个自然西施舌群体等位酶的遗传变异研究 |
| Allozyme Analysis of Genetic Diversity in five Natural Populations of Coelomactra antiguata from Coast of China |
| 投稿时间：2009-07-24 修订日期：2009-12-23 |
| DOI： |
| 中文关键词:西施舌，等位酶，遗传变异 |
| 英文关键词:Coelomactra antiquata, allozyme, genetic diversity |
| 基金项目:浙江省自然科学基金资助项目(1402039) |
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| 中文摘要: |
| 采用聚丙烯酰胺凝胶垂直平板电泳技术，研究了中国沿海的山东胶南（JN），江苏南通（NT），浙江台州（TZ），福建福州（FZ），广西北海（BH）西施舌（Coelomacatra antiquata)自然群体的等位基因酶遗传变体，对其斧足，外套膜和肝脏组织进行了13种同工酶的比较试验，最后选择肝脏作为西施舌的等位酶研究组织。选取谱带清晰的8种等位酶进行检测，共检测到17个位点。JN，NT，TZ，FZ, BH群体的多态位点比例分别是58. 82%，70. 59%， 50. 00%，56. 25 % ，41.17%，平均有效等位基因数（Ae)分别为1 .550，1 .555，1 .434，1 .602 ，1 .828，平均杂合度观察值（Ho)分别为0.240，0.270，0.239，0.243 ，0.288。结果表明，群体的遗传变异水平BH>NT>FZ>JN>TZ，中国沿海西施舌遗传变异水平较高，种质资源较好；同时发现浙江台州（TZ）群体外，普遍存在杂合子缺失现象；并根据等位酶资料对5个群体进行了ＵＰＧＭＡ聚类分析。 |
| 英文摘要: |
| Allozyme variation within five natural populations of Coelomactra antiquate from Jiaonan Shandong province(JN)，Nantong Jiangsu province(NT)，Taizhou Zhejiang province，(TZ)，Fuzhou Fujian province(FZ)，Beihai Guangxi province(BH) were investigated by vertical polyacrylamide gel electrophoresis. Thirteen isozymesin three different tissues(foot，mantle and liver) of Coelomactra antiquate were analyzed and compared. At last, choose liver as allozyme experiment's tissue. Seventeen loci of eight enzyme systems were detected and used for estimating population genetic diversity. The percentages of polymorphic loci in JN，NT，TZ，FZ and BH populations were 58. 82%，70. 59%， 50. 00%，56. 25 % and 41.17%，respectively. The mean effective numbers of allele were 1 .550，1 .555，1 .434，1 .602 and 1 .828，respectively. The mean heterozygosities observed were 0. 240，0. 270，0. 239，0. 243 and 0. 288，respectively. It means that the genetic variations were BH>NT>FZ>JN>TZ，the genetic diversity was high and germ plasma were good in five populations of Coelomactra antiquata. The heterozygous deficiency was common in all populations except for the TZ population. Based on the allozymic variation data，a UPGMA dendrogram was conducted in five populations. |
| 包永波,余冬冬,尤仲杰,林志华.2010.中国沿海5个自然西施舌群体等位酶的遗传变异研究[J].水生态学杂志,31(3):76-79. |
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