| ２种大眼蟹线粒体ＣＯＩ基因序列的比较研究 |
| Sequence Comparison of Mitochondrial COI Gene between Two Species of Macrophthalmid Crabs |
| 投稿时间：2009-06-07 修订日期：2009-07-06 |
| DOI： |
| 中文关键词:宽身大眼蟹 日本大眼蟹 ＣＯＩ 序列 |
| 英文关键词:Macrophthalmus dilatatum M. japonicus COI Sequence |
| 基金项目:重庆文理学院引进人才专项 |
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| 中文摘要: |
| 测定了宽身大眼蟹(Macrophthalmus dilatatum)和日本大眼蟹(M.japonicus)线粒体细胞色素氧化酶I亚基(COI)基因片段的序列,其长度均为658bp,A、T、G、C的含量宽身大眼蟹为27.4%、30.9%、17.3%、24.4%,日本大眼蟹为25.8%、32.0%、17.4%、24.7%。宽身大眼蟹只有1种单倍型,日本大眼蟹出现2种单倍型,2种间有123(124)个变异位点,核苷酸差异率为18.69%(18.85%),其中转换68(69)处,颠换55(55)处,转换与颠换比约为1.2(1.3);种间差异远大于种内个体间差异(0.61%)。2种大眼蟹的AT(57.9%)含量明显高于GC含量,与其他蟹类COI基因研究结果相似。系统发生树的拓扑结构支持大眼蟹科与弓蟹科亲缘关系相对较近,二者为姊妹群关系。 |
| 英文摘要: |
| Partial sequences of the mitochondrial COI gene were determined for two Macrophthalmus species(M.dilatatum and M. japonicus)，and the length of sequence of the two species was the same. of the 658 nucleotides obtained，the A，T，G and C contents were similar, the contents of M. dilatatum and the average contents of M. japonicus were 27.4% , 30.9%，17.3%，24 . 4 % and 25.8%，32.0%，17.4%，24.7% respectively. Three haplotypes were found in M. dilatatum and M. japonicus，the former was one and the latter was two. There were 123(124)variable sites between the two species，including 68(69)transition sites and 55 ( 55) transversion sites. The si/sv and ratio of sequence divergence were about 1.2(1.3) and 18.69%(18.85%) respectively. The intraspecific difference (0.61%)was less than the interspecific difference. The average AT content(57.9%)of the two Macrophthalmus species was higher than GC content，which was similar to the results of studies on other crabs. The topological structure of the molecular phylogenetic tree revealed that a close phylogenetic relationship between Macrophthalmidae and Varunidae，forming a sister group relationship.
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| 徐敬明.2010.２种大眼蟹线粒体ＣＯＩ基因序列的比较研究[J].水生态学杂志,31(2):42-47. |
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