| 二温式聚合酶链反应鉴别诊断迟缓爱德华氏菌病 |
| Identification of Edwarsiella tarda Strains by Two-temperature PCR |
| 投稿时间：2007-12-11 |
| DOI： |
| 中文关键词:二温式ＰＣＲ 鉴别 爱德华氏菌 迟钝爱德华氏菌 |
| 英文关键词:two-temperature PCR identification Edwardsiella EdwarsieUa tarda |
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| 摘要点击次数: 1906 |
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| 中文摘要: |
| 根据迟缓爱德华氏菌株２３ＳｒＤＮＡ基因，设计合成一对引物ＸＺＥ１５、ＸＺＥ１６，建立了二温式聚合酶链反应（ＰＣＲ）鉴别诊断迟缓爱德华氏菌株的技术。特异性试验表明，对３株迟钝爱德华氏菌株进行ＰＣＲ扩增出与预期大小相一致的２８４ｂｐ的特异性片段，但对３株钻鱼爱德华氏菌及其他对照鱼病病原体核酸模板的ＰＣＲ扩增不出现任何条带；敏感性试验结果显示，该二温式ＰＣＲ可以检测到１０ｐｇ的迟钝爱德华氏菌ＤＮＡ。
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| 英文摘要: |
| A pair of primer XZE15 and XZEI6 was designed and synthesized according to the conserved gene＇ sequences of 23S rDNA of Edwarsiella tarda , and then reaction parameters were optimized to develop two-temperature polymerase chain reaction （PCR） to simultaneously identify E. tarda. The results showed that only of 284pblong DNA fragment for three E. tarda strains was amplified, and no DNA fragments for three Edwarsiella ictalur strains and other fish pathogenic viruses and bacteria were amplified by this two-temperature PCR. And 10 pg DNA of E. tarda was detected by this two-temperature PCR. |
| 邓显文,谢芝勋,谢丽基,刘加波,谢志勤,庞耀珊,余晓丽.2009.二温式聚合酶链反应鉴别诊断迟缓爱德华氏菌病[J].水生态学杂志,30(2):158-160. |
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