| 水产品中三聚氰胺残留的测定研究 |
| Determination of Melamine Residue in Fishery Products |
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| DOI： |
| 中文关键词:水产品 三聚氰胺 高效液相 |
| 英文关键词:Fishery products Melamine High-performance liquid chromatography-tandem mass spectrometry |
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| 中文摘要: |
| 建立了水产品中三聚氰胺残留含量测定的液相色谱-串联质谱法(LC-MS/MS)。样品经1%三氯乙酸溶液和乙腈超声提取,MCX柱净化,在正离子电喷雾离子源(ESI+)、多反应监测方式(MRM)模式下进行测定。定性离子对为127/85和127/68;定量离子对为127/85。线性范围为10～400ng/mL,在添加水平为0.01～10.0mg/kg时的平均回收率为80.5%～94.1%,方法的定量限为0.01mg/kg。 |
| 英文摘要: |
| method for determination of melamine residue in fishery products by liquid chromatography-mass spectrum （LC-MS/MS）was established. The conditions of LC-MS/MS was optimized. The sample was ultrasonically extracted with 1% triehloroactic acid and ethylamine, and cleaned by the solid phase extraction （MCX） cartridge. The analyte was identified by electrospray ionization （ESI） in positive mode using multiple reaction monitoring （MRM）. The determination ion pairs are 127/85 and 127/68,and the confirmation ion pairs are 127/85. The method was validated and good results were obtained with respect to spiked recoveries. The linear range was from 10 to 400 ng/mL,the average recoveries were between 80.5%and 94.1% in the spiked rang of 0.01 -10.0 mg/ kg. LOQ was 0.01 mg/kg. |
| 梁丽军,张海琪,何中央,王丽丽,施礼科.2009.水产品中三聚氰胺残留的测定研究[J].水生态学杂志,30(6):113-117. |
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