| 杂交贻贝同工酶表达的初步分析 |
| Preliminary Analysis on the Isozyme Phenotype of Hybrid Mytilus |
| 投稿时间：2008-01-01 |
| DOI： |
| 中文关键词:厚壳贻贝 紫贻贝 杂交贻贝 同工酶 |
| 英文关键词:Mytilus coruscus Gould Mytilus edulis Linnaeus Hybrid Mytilus Isozyme |
| 基金项目: |
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| 中文摘要: |
| 运用聚丙烯酰胺凝胶电泳技术，对2种贻贝（厚壳贻贝、紫贻贝）稚贝及通过人工培育所得的二者的杂交后代稚贝同工酶表达状况进行分析。结果显示，所检测5种酶（SOD、MDH、ME、ATP、ADH）在上述3种贻贝中的表达极为相似，MDH在3种贻贝中除了迁移率相同的条带外，在紫贻贝中表达出迁移率较小、表达较弱的条带；SOD和ATP仅在厚壳贻贝中表达出比另外2种多1条迁移率最大的条带，ME则表现为紫贻贝少1条中间的条带，ADH在杂交贻贝和紫贻贝中表达完全相同，厚壳贻贝虽也表现为3条酶带，但迁移率明显不同。试验结果说明，厚壳贻贝和紫贻贝具有较近的亲缘关系，其杂交后代与二者均有一定的相似性，这与传统的分类方法所得的结论相一致。 |
| 英文摘要: |
| Five isoenzymes （ SOD, MDH, ME, ATP, ADH）, extracted from the mantellum of three species of Mytilus, Mytilus edulis Linnaeus and Hybrid Mytilus （ which were from the hybridization between Mytilus coruscus Gould and Mytilus edulis Linnaeus by artificial cultivation） ,were analyzed by polyacrylamide gel electrophoresis （PAGE）. The results showed that five isoenzymes were greatly similar in three species of Mytilus above-mentioned. There were the same MDH bands in the three Mytilus and anther weak band with low mobility ratio in Mytilus edulis; SOD and ATP showed four phenotypes in Mytilus coruscus Gould but three in Mytilus edulis Linnaeus and Hybrid Mytilus. Me was empty of middle phenotype in Mytilus edulis Linnaeus. ADH was the same in Mytilus edulis Linnaeus and Hybrid Mytilus, the three bands also appeared in Mytilus coruscus Gould but with obviously different mobility ratio. The expression of isoenzymes showed there was relative relationship between Mytilus coruscus Gould and Mytilus edulis Linnaeus, and hybrid Mytilus showed similarity with both of them. The conclusion was in accord with the result of traditionary taxonomy. |
| 刘慧慧,常抗美.2009.杂交贻贝同工酶表达的初步分析[J].水生态学杂志,30(3):111-114. |
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