| 鳜鱼SSR-PCR反应条件的优化探索 |
| Discussion about the Reaction Conditions of Microsatellite PCR in Siniperca chuatsi |
| 投稿时间：2007-12-24 |
| DOI： |
| 中文关键词:鳜 微卫星 反应条件 |
| 英文关键词:Siniperca chuatsi Microsatellite Reaction condition |
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| 中文摘要: |
| 以鳜（Siniperca chuatsi）基因组DNA为试验材料，研究MgCl2浓度、dNTPs浓度、TaqDNA聚合酶3个反应参数对微卫星PCR的影响，建立一套适合鳜微卫星PCR反应的最佳体系。结果表明，在25μL的反应体系中，Mg2＋、dNTPs、TaqDNA聚合酶3个参数的适宜浓度分别为1．2mmol／L、0．2μmol／L和0．04U／μL。PCR反应程序为94℃变性5min，94℃30s，56℃1min，72℃1min，共30个循环，最后72℃延伸5min。 |
| 英文摘要: |
| In this study, A microsatellite DNA PCR reaction system of Siniperca chuatsi was constructed by the optimization of the concentrations of Mg2＋ ,dNTPS and Taq DNA Polymerase. The results showed that： The optimalized PCR system with total volume 25 μL including MgCl2 at 1.2 mmoL/L, dNTP at 0.2 μmol/L, Taq DNA polymerase at 0.04 U/μL. The PCR reaction program with 5 minutes at 94 ℃ ,which is followed by 30 cycles ,each with 30 seconds at 94℃, 1 minute at 56 ℃, 1 minute at 72℃, and a final extension at 72 ℃ for 5 minutes. |
| 匡刚桥,吴青,郑曙明,陈建,鲁双庆.2009.鳜鱼SSR-PCR反应条件的优化探索[J].水生态学杂志,30(3):128-130. |
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