| 鲢即刻早期基因c-fos和c-jun的克隆及其序列分析 |
| Cloning and Sequence Analysis of Immediately Early Genes (c-fos &c-jun) in Hypophthalmichthys molitrix |
| 投稿时间：2013-04-17 修订日期：2013-06-05 |
| DOI： |
| 中文关键词:白鲢 c-fos c-jun 基因克隆 序列分析 |
| 英文关键词:Silver carp c-fos c-jun Gene cloning Sequence analysis |
| 基金项目:国家自然科学基金 |
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| 中文摘要: |
| 原癌基因c-fos和c-jun 属于即刻早期基因，在神经元兴奋传导和中枢神经系统活动过程中起着重要作用。通过ＲＡＣＥ技术首次克隆了鲢（Ｈｙｐｏｐｈｔｈａｌｍｉｃｈｔｈｙｓｍｏｌｉｔｒｉｘ）肝脏c-fos和c-jun基因，通过生物信息学方法预测分析了该基因的结构和功能。结果表明，鲢肝脏c-foscDNA 全长为1719bp，开放阅读框1059bp，编码３５２个氨基酸；ｃ-jun cDNA全长1931bp，其中开放阅读框９２７ｂｐ，编码３０８个氨基酸；二级结构预测可知，c-fos 包含２５．８５％的α-螺旋，１２．７８％的延伸链，１．４２％的β 折叠和５９．９４％的无规则卷曲；ｃ-ｊｕｎ包含３２．１４％的α-螺旋，５．５２％的延伸链，１．６２％的β-折叠和６０.７１％的无规则卷曲。分析其功能结构位点，发现在１０８～１７２aa 和２２７～２９１aa处有１个典型的亮氨酸拉链结构。通过构建氨基酸系统发育树，鲢与草鱼（Ｃｔｅｎｏｐｈａｒｙｎｇｏｄｏｎｉｄｅｌｌｕｓ）c-fos同源性高达９７％，与草鱼c-jun同源性高达９５％，而与哺乳动物同源性较低。 |
| 英文摘要: |
| The proto-oncogene c-fos and c-jun，which are the members of immediate early gene family，play an important role in neurons excitative conduction and in central nervous system activity. The gene c-fos and c-jun in the liver of Hypophthalmichthys molitrix were cloned for the first time by RACE，while the gene's structure and function were predicatively analyzed by bioinformatics method. The entire length of c-fos cDNA was 1 719 bp，which contained a 1 059 bp open reading frame encoding 352 amino acids. The entire length of c-jun cDNA was 1 931 bp，which contained a 927 bp open reading frame encoding 308 amino acids. By the secondary structure forecast，the c fos had 25.85 % of a-helix，12.78 % of extended strand，1.42% of β-sheet，59. 94% of random coil，and the c-jun had 32. 14% of a-helix，5.52% of extended strand，1.62% ofβ-sheet，60. 71% of random coil. By analyzing the function and structure sites of c-fos and c-jun ，a typical leucine zipper motif was respectively found in 108-172 aa and 227-291 aa. By constructing phylogenetic tree of amino acid，it was found that the homology of c-fos between Hypophthalmichthys molitrix and Ctenopharyngodon idellus was as high as 97%，while the homology of c-jun between them was as high as 95 %.But the homology with mammals were low.
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| 李园园,马军国,李效宇.2013.鲢即刻早期基因c-fos和c-jun的克隆及其序列分析[J].水生态学杂志,34(6):63-69. |
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