| 大鲵精液离体保存的初步研究 |
| Preliminary Study on Conservation of Andrias davidanus Semen in Vitro |
| 投稿时间：2010-10-12 修订日期：2010-12-06 |
| DOI： |
| 中文关键词:大鲵 精液 离体保存 |
| 英文关键词:Andrias davidanus semen conservation in vitro |
| 基金项目: 陕西省科学院基础应用研究项目(2009K-14) |
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| 中文摘要: |
| 针对大鲵(Andrias davidianus)性腺发育的不同步现象,为提高受精率,对其精液特性和保存条件进行初步研究。人工辅助采集精液,对精液量、酸碱度、密度、活力等基本特性进行常规测定,分别对保护液、光照、温度等保存条件进行了分组研究试验。结果表明,亲鲵的精液量最多可达12mL/kg,pH值6.7～7.0,呈微酸状态;精子密度215万～1075万/mL,精子活力为0.7～0.9。离体保存条件下,室温暗环境(20lx)较光环境(400lx)可延长存活时间0.5h,暗环境低温(0～4℃)较室温(21～22℃)可延长存活时间3d,低温暗环境下原精液中添加保护液比对照组精子存活时间延长6～12h。 |
| 英文摘要: |
| Gonadal development of Andrias davidanus not synchronized，semen characteristics and preservation conditions of A. davidanus were studied targeting the increase of fertility rate. Semina were collected by artificial assistance approach，and a regular assay was conducted on the basic features such as semen volume，pH，density，vitality，etc. Preservation conditions，namely，protection medium，illumination and temperature，were also studied. The results showed that semen volume of pro-male A. davidanus was up to 12 mL/kg，pH 6. 7一7. 0 of subacid condition ;and that the semen density was(2. 15一10.75)million/mL, sperm motility 0. 7一0. 9. In vitro conservation conditions，dark environment at ambient illuminance (20 lx).The result also indicated that the survival time of the semina was 0.5 h longer than those conserved in the luminous environment (400 lx);low temperature(0-4℃)could extend the survival time of the semina 3d longer than those at room temperature(21一22 ℃)in dark environment ;in the cold dark environment，semina with protection medium added in could extend the survival time 6一12 h longer than the control group. |
| 赵虎,张红星,王启军,陈玖安.2012.大鲵精液离体保存的初步研究[J].水生态学杂志,33(1):149-152. |
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