| 三角帆蚌不同组织基因组DNA提取效果比较 |
| Comparative Study of Extraction Genmonic DNA in different Tissues of Hyriopsis cumingii |
| 投稿时间：2009-07-14 修订日期：2009-09-28 |
| DOI： |
| 中文关键词:三角帆蚌 基因组ＤＮＡ 斧足 |
| 英文关键词:Hyriopsis cumingii DNA extraction Foot |
| 基金项目:湖北省自然科学基金资助项目（2008CDB104），湖北省教育厅科研项目（Q20081204） |
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| 中文摘要: |
| 利用平衡酚-氯仿法分别提取三角帆蚌(Hyriopsis cumingii)斧足、鳃、闭壳肌、性腺、肝脏、心脏中的基因组DNA,采用紫外分光光度测定法、琼脂糖凝胶电泳法、PCR扩增法分析三角帆蚌不同组织基因组DNA的提取质量。结果表明,三角帆蚌6个组织均能成功地提取基因组DNA,但提取的DNA质量存在差异;斧足中提取的DNA纯度、浓度最高,PCR扩增的条带最亮,因而是三角帆蚌提取DNA的最佳组织;其次是肝脏和闭壳肌,心脏和性腺提取的DNA较差。 |
| 英文摘要: |
| Freshwater pearl mussel，Hyriopsis cumingii，is the most economically important mussel species in China. Extraction of genomic DNA from preserved specimen is a key step to study phylogeny and molecular genetics. DNA extraction from different tissues of H. cumingii was studied and electrophoresis，OD260/OD280，DNA concentration，PCR amplification were used to check the quantity of DNA. This paper shows that the genomic DNA from different tissues of H. cumingii was extracted by saturated phenol-chloroform method. The results indicated that adductor muscle，foot，gill，gonad，liver and heart could be used to extract DNA easily and successfully. In addition，the value of OD260/OD280 for each DNA samples above mentioned was about 1 .8，and the quantity was 10-20 g per 100 mg. The better tissue of extration DNA was heart. Also，clear and bright products of PCR amplification revealed that the DNA might meet the needs of DNA molecular markers.
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| 许巧情,刘俊.2010.三角帆蚌不同组织基因组DNA提取效果比较[J].水生态学杂志,31(2):121-124. |
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