| 密云水库入库河流水体总细菌和反硝化菌群落组成与结构研究 |
| Microbial Community Structures of Total Bacteria and Denitrifying Bacteria in the Tributaries of Miyun Reservoir |
| 投稿时间：2017-11-10 修订日期：2018-11-15 |
| DOI：10.15928/j.1674-3075.2018.06.007 |
| 中文关键词:总细菌 反硝化菌 群落结构 密云水库 |
| 英文关键词:total bacteria denitrifying bacteria bacteria community structure Miyun Reservoir |
| 基金项目:北京市自然科学基金（J150001）；北京市优秀人才培养资助青年骨干个人项目（2014765000432G189） |
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| 中文摘要: |
| 研究密云水库入库河流水体中总细菌和反硝化菌的群落组成与差异性，为水库的安全管理与水质保障提供基础数据和科学依据。选择对密云水库水质影响最大的5条入库河流（潮河、白河、清水河、对家河和白马关河），对水体中的总细菌和氮循环功能微生物反硝化菌进行了聚合酶链式反应（PCR）扩增与高通量测序分析。5条入库河流中全细菌主要隶属于6个门，而以Proteobacteria（变形菌门）为优势种群；反硝化菌在门水平的分布则相对简单，主要以Proteobacteria和少量Deferribacteres（脱铁杆菌门）构成。不同入库河流全细菌的群落结构具有空间差异，其中水文特征较为接近的白河和潮河全细菌群落结构具有较高相似性，与其他3条入库河流群落差别较大；水体中全细菌的Shannon多样性和Chao1丰富度均表现出较高水平。氮磷污染较重的对家河和潮河反硝化菌群落结构比较类似，其他3条入库河流之间则相对分散；对家河全细菌和反硝化菌的多样性和丰富度表现出较低水平。清水河水体中的全细菌和反硝化菌群落结构都较少受到已分析的环境因子影响，可能与该河道的人工改造有关。河流水体中全细菌以及关键功能微生物群落特征受到河流的水文、形态和理化指标的综合影响，可以作为水体环境与生态状况的重要指示项目，在今后的水库水环境保护与管理中应予以重视。 |
| 英文摘要: |
| Miyun Reservoir is the largest surface drinking water source for Beijing City. Precipitation recharge and water diversion from south to north contribute, but inflow from tributaries is the primary source of water to Miyun Reservoir and the primary determinant of reservoir water quality. The microbial community of rivers is an important indicator of water quality and ecological succession. To provide basic data and a scientific basis for reservoir management and water quality protection, we analyzed the community structure of the total bacteria and denitrifying bacteria in Miyun Reservoir tributaries by polymerase chain reaction (PCR) amplification and high throughput sequencing technology. Correlation of the microbial community with environmental parameters was examined using principal component analysis (PCA). On April 19, 2016, water samples for microbial analysis were collected from five tributaries (Chaohe River, Baihe River, Qingshui River, Duijia River and Baimaguan River) and physicochemical parameters for each sampling site were determined. The bacteria collected belonged primarily to six phyla (Proteobacteria, Bacteroidetes, Cyanobacteria, Actinobacteria, Firmicutes, Verrucomicrobia), with absolute dominance by Proteobacteria in all five tributaries. Denitrifying bacteria sequences were in two phyla, Proteobacteria and a small fraction from Deferribacteres. The bacterial and denitrifying bacterial community structure presented obvious spatial variation. Baihe River and Chaohe River, with similar hydrological properties, displayed high similarity in bacterial composition and were very different from the other three rivers. The Shannon diversity and Chao1 richness of the bacterial communities in Baihe River and Chaohe River were high, but low in Duijia River. Duijia River and Chaohe River, with high pollutant loading of N and P, exhibited similar compositions of denitrifying bacteria. PCA results indicate that pH, TOC, NH3-N and Cl- were the primary environmental parameters affecting the bacterial community, while DO, Chl-a, TP and NH3-N were the primary environmental parameters affecting the denitrifying bacteria community. However, neither bacterial community correlated well with environmental variables in Qingshui River. The community structure of bacteria in the five tributaries is affected by a combination of hydrological and physicochemical properties and is a good indicator of water quality and trophic status. We recommend that bacterial monitoring be given more attention in reservoir management. |
| 李 垒,孟庆义,叶 飞,王 雨.2018.密云水库入库河流水体总细菌和反硝化菌群落组成与结构研究[J].水生态学杂志,39(6):44-51. |
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