| 低温对红耳龟血液指标及植物血凝素反应值的影响 |
| Effects of Low Temperature on Hematological Indices and Response to Phytohemagglutinin in the Red-eared Slider Turtle, Trachemys scripta elegans |
| 投稿时间：2022-06-13 修订日期：2022-07-14 |
| DOI：10.15928/j.1674-3075. 202206130225 |
| 中文关键词:红耳龟 低温 淋巴细胞 嗜酸性粒细胞 植物血凝素 应激 |
| 英文关键词:Trachemys scripta elegans low temperature lymphocytes eosinophils phytohemagglutinin stress response |
| 基金项目:安徽省舒城县“三区”科技人才项目（2021）；安徽农业大学2020年度省级重点教学研究项目（2020jyxm0531）和2021年省级线下课程项目（2021xxkc039）；安徽农业大学2021年国家级大学生创新创业训练计划项目创新训练项目（202110364013）。 |
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| 中文摘要: |
| 探究低温对红耳龟（Trachemys scripta elegans）免疫功能和应激反应能力的影响，可揭示红耳龟适应低温环境的生理学和免疫学基础，为深入了解其入侵生物学提供基础资料。采用活体背甲下静脉窦采血技术，测定了6℃、17℃和26℃条件下处理1 d、5 d和10 d时血液中各型白细胞百分比及嗜异性粒细胞heterophil granulocyte与淋巴细胞的比值（ratio of heterophils to lymphocytes，H/L比值），以及第10 d时采血后植物血凝素（phytohemagglutinin-P，PHA-P）反应的变化。结果显示：（1）随着温度下降，第1 d时只有嗜碱性粒细胞的百分比显著升高（P<0.05），此差异在第5 d时和第10 d时消失（P>0.05）；第5 d时，嗜酸性粒细胞的百分比和H/L比值先升后降，淋巴细胞的百分比先降后升，单核细胞的百分比下降（P<0.05）；第10 d时，嗜酸性粒细胞的百分比上升，淋巴细胞、嗜异性粒细胞和单核细胞的百分比，以及H/L比值下降（P<0.05）。（2）随着处理天数延长，除嗜碱性粒细胞的百分比无组内差异外（P>0.05），其他类型白细胞的百分比及H/L比值都有组内差异（P<0.05）。（3）6℃组、17℃组和26℃组对PHA-P的反应分别在注射后48 h、24 h和48 h达到峰值，但组间差异不显著（P>0.05）。红耳龟通过调整血液中嗜酸性粒细胞等不同类型白细胞的百分比、下降的应激反应能力和提前发生反应的免疫应答来适应低温的短期胁迫。 |
| 英文摘要: |
| In this study, we explored the effects of low temperatures on immune function and stress response in the red-eared slider turtle, Trachemys scripta elegans. Our aim was to reveal the physiological and immunological mechanisms of their adaptation to low-temperature environments, and provide basic data on their biological behavior as an invasive species. Healthy red-eared slider turtles purchased at the flower fair were exposed to three water temperatures (6 oC, 17 oC, and 26 oC) for 10 days, with 10 turtles in each group. On days 1, 5 and 10, blood samples were collected from the dorsal cervical sinus and body mass, carapace length and width were measured The percentage of different leukocytes and the ratio of heterophils to lymphocytes (H/L) were determined, and their response to the antigen, phytohemagglutinin-P (PHA-P), was measured on day 10 after collecting blood samples. Results were as follows: (1) Temperature and time had no significant effects on or interaction with body mass, carapace length and width. (2) On day 1, the percentage of basophils significantly increased at 6 oC and 17 oC (P<0.05), but this effect was not observed on day 5 and day 10 (P>0.05). On day 5, the percentage of eosinophils and H/L increased and then decreased, the percentage of lymphocytes initially decreased and then increased , while the percentage of monocytes tended to decrease (P<0.05). On day 10, the percentage of eosinophils increased, but the percentage of lymphocytes, heterophils and monocytes, and H/L decreased (P<0.05). (3) After 10 days, the percentage of leukocytes, except for the basophils and H/L, showed significant differences within temperature groups (P<0.05). (4) The response of the red-eared slider turtles to the PHA-P antigen peaked at 48 h, 24 h, and 48 h after injection in the 6 oC, 17 oC, and 26 oC groups, respectively, with no significant differences among the temperature groups (P>0.05). Red-eared slider turtles adapt to short-term low temperature stress by adjusting the proportions of different types of leukocytes, reducing stress response capacity, and altering immune response timing. |
| 张一帆,林涵语,李崧铭,伍 亮,刁迎珠,张志强.2024.低温对红耳龟血液指标及植物血凝素反应值的影响[J].水生态学杂志,45(4):148-154. |
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