| 敌百虫胁迫对克氏原螯虾免疫学相关指标的影响 |
| Effects of the Stress of Trichlorphon on Some Immune Parameters of Procambarus clarkii |
| 投稿时间：2013-11-19 修订日期：2013-12-31 |
| DOI： |
| 中文关键词:克氏原螯虾 敌百虫 胁迫 免疫因子 |
| 英文关键词:Procambarus clarkii trichlorphon stress immune parameter |
| 基金项目:山东省科技发展计划项目(2010GCG20901)；山东省海洋与渔业厅科技计划项目 |
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| 中文摘要: |
| 在不同质量浓度（0、0.132、0.264、0.528 mg/L）敌百虫胁迫条件下，对克氏原螯虾（Procambarus clarkii）肝胰腺T-SOD、CAT、ACP、AKP及肌肉Na+,K+-ATPase、TChE活性的影响进行了研究。结果表明，敌百虫对各免疫因子影响各异；0.132 mg/L 和0.528 mg/L浓度组克氏原螯虾的肝胰腺T-SOD活性分别于药浴4 d和1 d时显著高于对照组（P﹤0.05）；0.132 mg/L 和0.264 mg/L 浓度组克氏原螯虾的CAT活性于药浴2 d后显著高于对照组（P﹤0.05）；12 d内各浓度组ACP及AKP活性随药浴时间的延长而一直低于对照组，其中ACP在4 d内逐渐降低；肌肉TChE在0.132 mg/L 和0.264 mg/L 浓度组中分别于药浴1 d和4 d后活性显著高于对照组（P﹤0.05）；而Na+,K+-ATPase只有0.528 mg/L 浓度组在2 d内活性显著高于对照组（P﹤0.05），其余各浓度组未出现诱导现象；试验组各免疫因子活性在药浴12 d时均明显低于对照组（P﹤0.05）；表明敌百虫胁迫并非仅仅作用于TChE， 其对机体T-SOD、CAT、ACP、AKP及Na+,K+-ATPase均有影响，12 d后各免疫因子活性均难以恢复至对照水平。
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| 英文摘要: |
| To investigate the influence of trichlorphon stress on the immune parameters of Procambarus clarkii, Procambarus clarkii for the experiment were reared in the test solution of 0 mg/L, 0.132 mg/L,0.264 mg/L and 0.528 mg/L trichlorphon for 12 days, respectively. The activities of four nonspecific immune parameters (T-SOD,CAT,ACP and AKP) in hepatopancreas and two in muscle (Na+,K+-ATPase and TChE) were measured．The results showed that the effects of trichlorphon on the immune parameters were different. The activity of T-SOD increased significantly after exposure to 0.132 mg/L trichlorphon for 4 days and 0.528 mg/L trichlorphon for 1 day. The activity of CAT was significantly higher compared with the control group at the concentration of 0.132 mg/L and 0.264 mg/L after 2 days. The activities of ACP and AKP were always lower than those of the control group during the period of 12 days in all groups, and ACP activity decreased continuously from 1 d to 4 d．The activity of TChE in muscle increased at the concentration of 0.132 mg/L and 0.264 mg/L trichlorphon after 1 day and 4 days, respectively. No difference in Na+,K+-ATPase activity was observed except the test group of 0.528 mg/L trichlorphon with Na+,K+-ATPase activity significantly higher than that of the control group within 2 days（P﹤0.05）. The study suggested that the immune parameters of Procambarus clarkia in each test group were significantly lower than those of the control group after 12 days（P﹤0.05）and they couldn’t restore to the normal level.
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| 钟君伟,安丽,孟庆磊,张龙岗,朱永安.2014.敌百虫胁迫对克氏原螯虾免疫学相关指标的影响[J].水生态学杂志,35(2):95-100. |
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