| 氨氮对泥鳅的急性毒性及对其肝、鳃组织超微结构的影响 |
| Acute Toxicity Test of Ammonia Nitrogen and Effects of Ammonia-N Stress on the Ultrastructure of Gill and Liver of Misgurnus anguillicaudatus |
| 投稿时间：2012-09-21 修订日期：2012-10-16 |
| DOI： |
| 中文关键词:泥鳅 氨氮胁迫 肝脏 鳃 超微结构 |
| 英文关键词:Misgurnus anguillicaudatus ammonia-N stress liver gill ultrastructure |
| 基金项目: 江苏省科技厅项目(BE2009334);苏州市科技局项目(SNG0822) |
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| 中文摘要: |
| 通过急性毒性和胁迫试验,探讨氨氮对泥鳅(Misgurnus anguillicaudatus)的急性毒性及对其肝、鳃超微结构的影响,为其养殖水质指标管理提供理论依据。急性毒性试验结果表明,在水温(25±1)℃、pH(8.1±0.1)、溶解氧(6.5±0.5)mg/L时,水中氨氮浓度越高,泥鳅的死亡率越高,其96 h LC50总氨浓度(TAN)为164.4 mg/L,非离子氨(NH4+-N)浓度为2.22 mg/L。胁迫试验结果表明,随着氨氮浓度的变化,试验组泥鳅鳃组织在21 d后出现不同程度的变化;其中,50 mg/L和150 mg/L浓度组的泥鳅鳃丝表面出现分泌物,细胞水肿;250 mg/L组鳃丝表面分泌物增加,鳃小片聚拢,上皮细胞水肿加剧;450 mg/L组鳃上皮坏死。泥鳅肝脏细胞超微结构在不同氨氮浓度下也出现不同程度的损伤,50 mg/L和150 mg/L组的泥鳅肝脏细胞线粒体扭曲变形,细胞核变形,核膜肿胀;250 mg/L组肝脏细胞胞浆空泡化,残存细胞器的结构不完整;450 mg/L组出现大量肝脏细胞凋亡。随着氨氮胁迫浓度的不断升高,鳃组织和肝细胞超微结构的受损程度逐渐严重,且损伤不可逆转。 |
| 英文摘要: |
| In order to provide scientific theoretical basis for water quality management during loach (Misgurnus anguillicaudatus ) culture，an acute toxicity test of ammonia nitrogen and ammonia-N stress on the microstructures of gill and liver of loath were studied. Results of acute toxicity test showed that under water temperature(25 ± 1)℃，pH(8. 1 ± 0. 1)，dissolved oxygen ( 6. 5 ± 0. 5)mg/L, the higher the concentration of water ammonia nitrogen，the higher the motality of loach; 96 h LC50 of total ammonia concentration(TAN)and nonionic ammonia(NH+4一N)were respectively 164.4 mg/L and 2.22 mg/L. Results of the stress test showed that with the ammonia-N concentration increased，loach gills appeared different levels of injuries after 21 d. In 50 mg/L and 150 mg/L groups，the gills of loach appeared secretions and cells edema. In 250 mg/L group，secretion on surface of gill filaments increased，gill lamellae gathered together and edema of epithelia cells was sharpen. In 450 mg/L group，there was cell necrosis of gills. The ultrastructure of loach liver cell under different concentrations of ammonia nitrogen appeared varying degrees of damage. In 50 mg/L and 150 mg/L groups，mitochondria distorted，and nuclei deformed with nuclear membrane swelling. In 250 mg/L group，liver cell structure was badly damaged，and there was cytoplasm vacuolization and incomplete structure of the organelles. In 450 mg/L group，massive liver cells appeared apoptosis. With ammonia-N concentration increased，the damage in gill tissue and liver cells seemed gradually serious，and the damage was irreversible. |
| 郝小凤,刘洋,凌去非.2012.氨氮对泥鳅的急性毒性及对其肝、鳃组织超微结构的影响[J].水生态学杂志,33(5):101-107. |
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