| 鳗弧菌感染对日本鑚部分免疫活性因子的影响 |
| Changes of Some Immunoactive Enzymes in Charybdis japonica Infected with Vibrio parahemolyticus |
| 投稿时间：2010-12-23 修订日期：2010-12-23 |
| DOI： |
| 中文关键词:日本蟳 鳗弧菌 免疫活性 |
| 英文关键词:Charybdis japonica (A. Milne-Edwards) Vibrio parahemolyticus immunoactive |
| 基金项目: “十一五”国家科技支撑计划重大项目(2006BAD09A01);江苏省海洋生物技术重点实验室开放基金(2007HS016) |
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| 中文摘要: |
| 对日本蟳(Charybdis japonica)进行鳗弧菌(Vibrio anguillarum)悬液和生理盐水(对照)注射感染,研究其血清免疫因子超氧化物歧化酶(SOD)、酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、过氧化氢酶(CAT)活性的变化,并对试验结果进行统计学分析。结果表明,注射鳗弧菌悬液组SOD活性12、24、48h均高于对照组,48h达到最高;CAT活性12、24h增加非常明显,12h达最高;AKP活性5、12、24h都有增加,均高于对照,24h最高;ACP活性12h开始上升,24h达最高。在人工感染鳗弧菌48h内,日本蟳体内的免疫因子发生了明显的变化,血清中SOD、ACP、AKP、CAT活性在不同时间段均有显著升高趋势,高于对照组水平。 |
| 英文摘要: |
| Charybdis japonica (A. Milne-Edwards)was infected with Vibrio anguillarum through injection，using normal saline as parallel control. The variations in activities of serum immunity factors including superoxide dismutase(SOD )，acid phosphatase(ACP)，alkaline phosphatase(AKP)，peroxide catalase(CAT) were assayed. Statistical analysis showed that SOD activity in the infected C. japonica increased in 12 h，24 h，48 h after injection，higher than that in the control group，and reached maximum in 48 h. The CAT activity increased significantly and reached maximum in 12 h. The AKP activity increased in 5 h，12 h，24 h，higher than that in the control group，and reached maximum in 24 h. The ACP activity begun to rise in 12 h，and reached highest activity in24 h. The results indicated that in 48 h of artificial V. anguillarum infection，sero-immunity factors in C. japonica varied significantly. The activities of SOD，ACP，AKP，and CAT in blood serum increased significantly in different time periods and were higher than that in the control group. |
| 王洪斌,成明,吴琴,李士虎,阎斌伦.2012.鳗弧菌感染对日本鑚部分免疫活性因子的影响[J].水生态学杂志,33(3):112-115. |
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