| 扁吻鱼和塔里木裂腹鱼线粒体ＣＯＩ基因片段的比较研究 |
| Comparative Study on Partial Mitochondrial COI Gene of Aspiorhynchus laticeps and Schizothorax biddulphi |
| 投稿时间：2010-05-21 修订日期：2010-08-16 |
| DOI： |
| 中文关键词:扁吻鱼 塔里木裂腹鱼 线粒体COI基因 |
| 英文关键词:Aspiorhynchus Laticeps Schizothorux biddulphi mtDNA COI gene |
| 基金项目:新疆维吾尔自治区自然科学基金项目(2009211A30);国家自然科学基金(31060356) |
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| 中文摘要: |
| 对扁吻鱼(Aspiorhynchus laticeps)和塔里木裂腹鱼(Schizothoraxbiddulphi)细胞色素c氧化酶亚基(COI)基因片段进行扩增。通过序列测定得到624bp的基因片段,无碱基的插入和缺失,A、T、G、C碱基中G含量最低,A+T含量高于G+C含量,与其它鱼类COI基因片段研究结果相一致。2个种间共检测到38处核苷酸替换,其中35处碱基转换,3处碱基颠换,蛋白质编码基因上的核苷酸替代主要是密码子第3位点上的同义替换。在编码的208个氨基酸中,仅发生2处氨基酸替代,替代率为0.96%。采用Kimura-2法计算种间平均遗传距离为0.06,以鲤作外群构建NJ系统树,扁吻鱼和塔里木裂腹鱼亲缘关系较近,而与新疆裸重唇鱼、斑重唇鱼亲缘关系较远。 |
| 英文摘要: |
| Mitochondrial cytochrome oxidase subunit I (COI) fragments of Aspiorhynchus Laticeps and Schizothorax biddulphi were amplified. By sequencing，the sequence of 624 bp nucleotide was obtained，and no insertion/deletion was observed. Base G content was the lowest of all，and the content of A+T was higher than that of G+C，which was similar to the results in other fishes. 38 nucleotide substitutions were checked between the two species which included 35 transitions and 3 transversions，and most of them were synonymous transitions at the third codonpositions in the protein-coding genes. There were only two amino acid substitution in encoded 208 amino acids，andthe substitution ratio was 0. 96%. The average genetic distance by Kimura-2 method between A. Luticeps and S.biddulphi was 0. 06 Cyprinus cyprio was chosen as an out-group，and the NJ clustering tree indicated that A. Laticeps and S. biddulphi had the closest relationship. |
| 杨天燕,张人铭,郭 焱,孟 玮,海 萨,马燕武.2011.扁吻鱼和塔里木裂腹鱼线粒体ＣＯＩ基因片段的比较研究[J].水生态学杂志,32(1):45-50. |
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