| 利用微卫星标记评估乌江彭水水电站对泉水鱼的遗传多样性影响 |
| Using Microsatellite Markers to Assess the Impacts by Pengshui Hydropower Station on Wujiang River on Population Genetic Diversity in Pseudogyrincheilus procheilus |
| 投稿时间：2009-02-28 |
| DOI： |
| 中文关键词:微卫星标记 水电站 泉水鱼 遗传多样性 |
| 英文关键词:microsatellite markers, hydropower station, Pseudogyrincheilus procheilus, genetic diversity |
| 基金项目: |
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| 中文摘要: |
| 乌江彭水水电站建成后形成阻隔，可能对生境连通性及坝上坝下鱼类的交流产生不利，影响到物种遗传多样性和群体遗传结构。本研究通过磁珠富集法构建了泉水鱼（Ｐｓｅｕｄｏｇｙｒｉｎｃｈｅｉｌｕｓｐｒｏｃｈｅｉｌｕｓ）微卫星文库，建立了泉水鱼微卫星ＤＮＡ分子标记体系，并利用这些微卫星分子标记初步评估了乌江彭水水电站对泉水鱼的遗传多样性影响。用６对具多态性的引物对乌江中３５尾野生泉水鱼样本进行遗传多样性分析，结果显示，６个位点共检
测到２８个等位基因；每个位点平均杂合度为０．６５３６，平均多态信息含量为０．６０８５，平均Ｓｈａｎｎｏｎ多样性指数为１.２７５２；卡方检验得出２个位点显著偏离Ｈａｒｄｙ－Ｗｅｉｎｂｅｒｇ平衡，且其中１个位点中出现了无效等位基因。实验初步表明乌江流域泉水鱼的遗传多样性较为丰富，但可能受到了水电站阻隔的干扰。
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| 英文摘要: |
| Fragmentation of the Wujiang river ecosystem caused by Pengshui hydropower station may disrupt the connectivity of ecosystem and communication of fish populations, consequently, fish population genetic structure and diversity will be affected. In this study, we established a partial genomic library for Pseudogyrincheilus procheilus. 6 microsatellite markers were assessed genetic diversity for 35 individuals. The results show that the total num- bers of alleles in 6 loci was twenty - eight. The average heterozygosity （ H ）, average polymorphism information content （PIC） and average Shannon＇s Information index （I） were 0. 6536, 0. 6085 and 1. 2752, respectively. Two loci showed significant deviation from Hardy-Weinberg equilibrium, and one of them had evidence for null alleles. The fndings indicated that although the genetic diversity of P. procheilus in Wujiang river was rich, it may had been infuenced by Pengshui hydropower station to a certain extent. |
| 史方,徐念,熊美华,王翔,杨钟,阙延福,朱滨,胡菊香,常剑波.2009.利用微卫星标记评估乌江彭水水电站对泉水鱼的遗传多样性影响[J].水生态学杂志,30(2):117-121. |
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