| 5种配方和5种糖类对大鳞副泥鳅精子保存效果的研究 |
| Study on Effects of Five Preservative Fluids and Five Carbohydrates on the Preservation of Paramisgurnus dabryanus Spermatozoa |
| 投稿时间：2008-05-04 |
| DOI： |
| 中文关键词:大鳞副泥鳅 精子活力 保存液 糖类 影响 |
| 英文关键词:Paramisgurnus dabryanus Spermatozoa motility Preservative fluids Carbohydrates Effects |
| 基金项目: |
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| 中文摘要: |
| 选择大鳞副泥鳅（Paramisgurnus dabryanus）进行人工采精，对精子进行保存，比较5种精子保存液（配方A、B、C、D、E）、5种糖及室温（20～24℃）和4℃对精子活力的影响，研究其精子保存的最佳条件，为规模化苗种的人工繁殖和杂交育种提供理论依据。结果显示，精子保存液保存的时间：C〉B〉A〉E〉D；精子保存液C、B、A、E、D分别能使精子在132h、132h、72h、22h、6h内使80％的精子保持快速运动的能力。在不同糖类进行比较的过程中，发现加入糖后的精子保存液比未加的保存效果要好，但不同糖类的效果又有差别，保存的时间为：葡萄糖〉蔗糖〉L-山梨糖〉D-果糖〉D-木糖。对20～24℃保存与低温4℃保存的效果研究表明，室温保存效果远不如冰箱低温保存效果。大鳞副泥鳅精子在淡水中快速运动时间能持续约31s，寿命约为130S。 |
| 英文摘要: |
| Chose big, healthy male Paramisgurnus dabryanus and collected spermatozoa artificially from the fish, preserved them according to demands, to find out the effects of five preservative fluids,five carbohydrates and different temperatures, thus, to get more information about the preservation of Paramisgurnus dabryanus spermatozoa, and provide basic knowledge for the artificial propagation of fingerlings and Paramisgurnus dabryanus cross breeding. The results showed that effects of five preservative fluids were different, the order of preservation time length was C 〉 B 〉 A 〉 E 〉 D. The preservative fluid C、B、A、E、D can keep 80% of spermatozoa having fast motility within 132h,132h,72h,22h,6h respectively. The results of comparison among different carbohydrates showed that the preservative fluid C which had been added carbohydrate was generally better than the former C. In the experiments of preservation, the best carbohydrate for spermatozoa preservation was Glucose, then was Saccharose, but the difference between them was not obvious, next was L- Sorbose, next was D -Fructose, the last one was D -Xylose. And the results of different temperature preservation experiment showed that the low temperature preservation （d℃） was much better than room temperature（20 -2d℃ ） preservation. The fast moving time in fresh water of Paramisgurnus dabryanus spermatozoa was 31 s. The life span in fresh water of Paramisgurnus dabryanus spermatozoa is 130 s. |
| 万全,李飞,王冰.2009.5种配方和5种糖类对大鳞副泥鳅精子保存效果的研究[J].水生态学杂志,30(5):85-89. |
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