附石生物营养质量对干旱胁迫的响应及机制
Response Mechanism and Nutritional Quality in Epilithon under Drought Stress
投稿时间:2024-01-12  修订日期:2024-02-29
DOI:10.15928/j.1674-3075.202401120013
中文关键词:干旱胁迫  附石生物  脂肪酸  营养质量  宏基因组
英文关键词:drought stress  epilithon  fatty acid  nutritional quality  metagenome
基金项目:国家自然科学基金项目(32271665、32030069)
作者单位
李唤唤 西藏大学生态环境学院西藏 拉萨 850000 
谭香 中国科学院武汉植物园水生植物与流域生态重点实验室湖北 武汉 430074中国科学院&湖北省丹江口湿地生态系统野外科学观测研究站湖北 武汉 430074 
张全发 中国科学院武汉植物园水生植物与流域生态重点实验室湖北 武汉 430074中国科学院&湖北省丹江口湿地生态系统野外科学观测研究站湖北 武汉 430074 
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中文摘要:
      探讨河流附石生物营养质量对干旱的响应及机制,为河流生物膜研究和对全球气候变暖大背景下的脆弱水域保护提供科学支持。基于脂肪酸和化学计量学2种食物营养质量指示方法,利用中宇宙控制实验模拟野外干旱情景,研究了干旱对附石生物营养质量的影响,并从宏观的附石生物膜藻类群落结构和微观的宏基因组技术2个层面探索其影响机制。结果表明,干旱后多不饱和脂肪酸(Polyunsaturated fatty acid,PUFA)中的二十二碳六烯酸(Docosahexaenoic acid,DHA)和二十碳五烯酸(Eicosapentaenoic acid,EPA)等含量减少;附石生物中磷(P)含量显著减少(P<0.05),C/P、N/P比值增加。硅藻是附石生物膜中的优势类群,优质脂肪酸的生产者,富含EPA、DHA,干旱后硅藻密度和丰度显著减少(P<0.05)。基于宏基因组分析结果,附石生物脂质代谢通路中脂肪酸生物合成显著减少(P<0.05),脂肪酸降解显著增加(P<0.05),进而影响EPA、DHA的含量。干旱导致河流生态系统基础资源营养质量下降,进而可能对整个河流生态系统中消费者的生长和繁殖产生影响。研究结果从基础资源碳质量新视角为水生态系统食物网的碳源及传递研究提供了重要依据。
英文摘要:
      Against the backdrop of global warming, droughts are happening more often. The epilithon is crucial to primary production in river ecosystems, forms the cornerstone of material circulation in the food web, and provides a carbon source for higher-level consumers. The decline in water area during droughts inevitably affects the epilithon habitat. Yet, the impact of drought on the nutritional quality of epilithon remains largely unexplored. In this study, we assessed the impact of drought on nutritional quality by conducting a mesocosm manipulation experiment, focusing on stoichiometry and polyunsaturated fatty acids (PUFA), particularly eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). The response and mechanism of nutritional quality in epilithon was then analyzed in terms of the content and composition of fatty acids, algae community structure, ecological stoichiometry, and microbial community using metagenomic sequencing. The mesocosm manipulation experiment included a drought treatment group and a control group and was run in triplicate. After preculturing for two weeks, an extreme drought was simulated in the treatment mesocosm, while water circulation was maintained in the control group. Epilithon samples were collected on day 0, 7 and 14 of the experiment for analysis of nutritional quality. This study not only affirmed the observed influence of drought on the nutritional quality of epilithon but also delved into the underlying mechanism by examining community composition and function of epilithon. The findings revealed a significant reduction in DHA and EPA content in epilithon in the drought treatment group. The phosphorus (P) content decreased significantly (P<0.05), resulting in an increase in the C/P and N/P ratios. Diatoms, the predominant epilithon group, rich in EPA and DHA, exhibited a significant decline in density and abundance after the drought (P<0.05). Additionally, metagenomic analysis revealed a significant reduction in fatty acid biosynthesis within the lipid metabolic pathway of epilithon (P<0.05), accompanied by a notable increase in fatty acid degradation (P<0.05), contributing to the overall decline in EPA and DHA. The study indicates that drought decreases the nutritional quality of a fundamental resource in river ecosystems, potentially influencing the growth and reproductive patterns of consumers in the food web. This study provides a novel perspective on the carbon quality of basal resources and sheds light on the intricate dynamics of food source and nutrient transfer within aquatic ecosystems.
李唤唤,谭香,张全发.2024.附石生物营养质量对干旱胁迫的响应及机制[J].水生态学杂志,45(3):21-31.
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