达里湖高原鳅不同地理群体遗传多样性与遗传分化研究
Genetic Diversity and Population Genetic Differentiation Analysis of Triplophysa dalaica in Different Populations
投稿时间:2022-06-13  修订日期:2022-08-30
DOI:
中文关键词:达里湖高原鳅  遗传多样性  遗传分化  种群历史动态
英文关键词:Triplophysa dalaica  genetic diversity  population differentiation  demographic history dynamics
基金项目:山东省自然科学基金(ZR2021QC224);枣庄学院博士科研启动基金(1020737)
作者单位E-mail
巩政 枣庄学院生命科学学院 zgongz@163.com 
王梓璇 枣庄学院生命科学学院 1981038953@qq.com 
张艺弛 西安理工大学西北旱区生态水利国家重点实验室 2210421249@stu.xaut.edu.cn 
赵耿楠 西安理工大学西北旱区生态水利国家重点实验室 921091813@qq.com 
潘保柱* 西安理工大学西北旱区生态水利国家重点实验室 zhuzipan@xaut.edu.cn 
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中文摘要:
      为了解达里湖高原鳅不同群体遗传变异现状,本研究选择线粒体Cyt b基因作为分子标记,分析了红碱淖、大黑河、辉腾河及漳河4个群体的遗传多样性、遗传分化及种群历史动态。结果表明,67尾个体中共检测到16个变异位点和12个单倍型;不同群体的单倍型多样性范围为0.233~0.744,核苷酸多样性范围为0.00020~0.00860。其中,查干诺尔湖流域的辉腾河群体遗传多样性水平最高;黄河流域的红碱淖与大黑河群体单倍型多样性水平较高,核苷酸多样性水平较低;海河流域的漳河群体单倍型多样性与核苷酸多样性均较低。单倍型系统发育树和进化网络图显示,12个单倍型未聚类形成明显的谱系结构,多数单倍型为单一群体独有;除漳河群体外,其群体的单倍型混杂连接,未遵循各自的地理分布格局。遗传分化分析结果显示,群体间遗传分化指数范围为0.233~0.904,多为高度分化;分子方差分析表明遗传变异主要来源于群体间(66.31%)。种群历史动态分析未检测到达里湖高原鳅各地理群体及总体在近期经历过种群扩张。鉴于达里湖高原鳅已成为雅鲁藏布江外来鱼类,建议将达里湖高原鳅自然分布区内的各群体划定为不同保护单元并跟踪监测栖息地质量,同时加强对物种交易和放生活动的管控。
英文摘要:
      Triplophysa dalaica, a small-sized loach of family Cobitidae, which was distributed in the mainstem and tributaries of the Yellow River downstream the Lanzhou reach and certain inner-flowing drainages in mid-eastern Inner Mongolia. So far, research on the population genetics of T. dalaica was very scarce. Therefore, it is necessary to examine the genetic diversity, population genetic differentiation, and demographic history dynamics of T. dalaica for species conservation and management. In this study, mitochondrial Cyt b gene sequences of 67 specimens from four locations were determined to perform the population genetics analyses. The results showed that a total of 16 polymorphic loci and 12 haplotypes were determined from four populations. The haplotype diversity index (Hd) and nucleotide diversity index (Pi) of four populations ranged from 0.233 to 0.744 and from 0.00020 to 0.00860, respectively. The genetic diversity of Huiteng River population (Chagannuoer Lake basin) was the highest. The haplotype diversity of Hongjiannao and Daheihe populations (Yellow River basin) was higher, but nucleotide diversity was lower. The haplotype diversity and nucleotide diversity of Zhanghe population (Haihe River basin) were both lower. The phylogenetic tree and evolution network of haplotypes showed they did not cluster into distinct lineages, and most haplotypes were unique to a single population. Meanwhile, these haplotypes mixed without obvious geographical distribution pattern in addition to two basal haplotypes of Zhanghe population. Genetic differentiation analysis indicated that most pairwise population were highly differentiated with the genetic differentiation index (FST) ranging from 0.233 to 0.904; analysis of molecular variance (AMOVA) demonstrated that a high proportion of the total genetic variance was attributable to variations between populations (66.31%). The demographic history dynamic analysis did not detect the population expansion of T. dalaica occurred in recent period. Besides, a specimen of T. dalaica was collected in the shannan reach of the Yarlung Tsangpo River during an ichthyological survey, which was the first record of T. dalaica as the alien species in the Yarlung Tsangpo River. In conclusion, the genetic diversity of T. dalaica varied in different populations, and the genetic differentiation among different populations were generally very high. Based on these research findings, we suggest that populations of T. dalaica in its natural distribution area should be treated as different protection units and their habitat quality should be monitored, whereas regulation of species trade and release should be strengthened.
巩政,王梓璇,张艺弛,赵耿楠,潘保柱.2024.达里湖高原鳅不同地理群体遗传多样性与遗传分化研究[J].水生态学杂志,45(4):92-98.
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