| 盐碱水中地衣芽孢杆菌对铜绿微囊藻生长抑制研究 |
| Growth Inhibition of Microcystis aeruginosa by Bacillus licheniformis in Saline-Alkaline Water |
| 投稿时间：2022-04-14 修订日期：2023-11-20 |
| DOI：10.15928/j.1674-3075.202204140116 |
| 中文关键词:地衣芽孢杆菌 铜绿微囊藻 藻类抑制 无菌滤液 盐碱水 |
| 英文关键词:Bacillus licheniformis Microcystis aeruginosa algae inhibition cell-free filtrate saline-alkaline water |
| 基金项目:国家重点研究计划(NO.2020YFD0900400)；中央级公益性科研院所基本科研业务费专项(2021XT04，2020TD52) |
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| 中文摘要: |
| 盐碱地养殖池塘极易爆发铜绿微囊藻(Microcystis aeruginosa)并危害养殖生物，基于菌藻平衡原理控制蓝藻是较为有效的措施之一，可降低铜绿微囊藻对养殖生物的危害。在实验室条件下，研究了盐碱水中地衣芽孢杆菌(Bacillus licheniformis)对铜绿微囊藻生长的抑制效果并探索其抑藻途径。结果显示，在碳酸盐碱度6.4 mmol/L、盐度3.5、pH 8.9的盐碱水中，地衣芽孢杆菌对初始密度为1.0×106、6.0×106、20×106 个/mL的铜绿微囊藻均有明显抑制作用，抑制率分别达到51.8%、65.7%、71.1%；地衣芽孢杆菌无菌滤液对铜绿微囊藻生长也表现出显著的抑制效果，其中无菌滤液加入量为实验水体2%时的抑制量最高，培养6d 后藻密度仅为4.5×106个/mL，处理组叶绿素a含量较对照组降低了58.8%，铜绿微囊藻光合作用相关基因PSBA1和PSBD1基因表达下调，光合作用途径受到抑制；盐碱水中地衣芽孢杆菌对铜绿微囊藻具有较好的抑制效果。研究结果可为盐碱地水产养殖中蓝藻控制提供理论支撑。 |
| 英文摘要: |
| Cyanobacteria such as Microcystis aeruginosa are the primary source of algal blooms in saline-alkaline ponds and endanger aquaculture organisms. Microbe-based algae control has attracted attention because of potential effectiveness, species specificity, and eco-friendliness. Previous research has shown that Bacillus licheniformis can inhibit M. Aeruginosa growth in fresh water. In this study, the growth inhibition of M. aeruginosa by B. licheniformis and its inhibition mechanism were investigated under laboratory conditions. We aimed to provide basic data for the control of cyanobacteria blooms in saline-alkaline aquaculture and propose a feasible method for regulating the quality of saline-alkaline water. In this study, there were two groups of experiments. One group was set as follows: three treatments with initial M. aeruginosa concentrations of 1×106, 6×106, and 20×106 cells/mL, and an initial concentration of B. licheniformis of 7.1×106 CFU/mL. The second group of experiments was set as follows: three treatments with an initial M. aeruginosa concentration of 1×106 cell/mL, and cell-free filtrates of B. licheniformis solution at concentrations of 0.5%, 1% and 2%. Each treatment was run in triplicate with a control, and the experiment lasted seven days. Cell density and absorbance of M. aeruginosa in each treatment was determined every three days. Growth of M. aeruginosa was significantly inhibited by B. licheniformis in saline-alkaline water under experimental conditions: carbonate alkalinity, 6.4 mmol/L; pH, 8.9 and salinity, 3.5. The inhibition rates of M. aeruginosa at initial M. aeruginosa concentrations of 1×106 cells/mL, 6×106 cells/mL and 20×106 cells/mL were, respectively, 51.8%, 71.1% and 65.7%, with normal M. aeruginosa growth in the control. The cell-free filtrate of B. licheniformis also showed a strong inhibitory effect on the growth of M. aeruginosa, and was most pronounced at the highest concentration (2%)，with the M. aeruginosa concentration of 4.5×106 cell/mL after culture for 6 d . At a filtrate concentration of 2%, the chlorophyll-a content in the experimental group was 58.8% lower than that in the control group, a significant reduction. B. licheniformis affected the expression of key M. aeruginosa genes involved in photosynthesis, such as PSBA1 and PSBD1, with significant downward regulation. In conclusion, B. licheniformis was found to inhibit M. aeruginosa photosynthesis in saline-alkaline water, consequently inhibiting growth. |
| 周成夷,么宗利,来琦芳,袁春营,高鹏程,陆建学,李 燕,周 凯,刘一萌,孙 真.2023.盐碱水中地衣芽孢杆菌对铜绿微囊藻生长抑制研究[J].水生态学杂志,44(6):136-141. |
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