增温对黑斑侧褶蛙蝌蚪形态表型和生理特征的影响
Effects of Increased Temperature on Morphological Phenotypes and Physiological Traits of Pelophylax nigromaculatus Tadpoles
投稿时间:2022-03-01  修订日期:2022-03-31
DOI:10.15928/j.1674-3075.202203010054
中文关键词:黑斑侧褶蛙  水温  形态表型  变态时长  生理特征  应激反应
英文关键词:Pelophylax nigromaculatus  water temperature  morphological phenotype  metamorphosis duration  physiological traits  stress response
基金项目:安徽农业大学2020年度省级重点教学研究项目(2020jyxm0531)和2021年度省级线下课程项目(2021xxkc039);安徽农业大学2021年校级(XJDC202110364053)和省级(S202110364019)大学生创新创业训练项目创新训练项目
作者单位
裴鑫怡 安徽农业大学动物科技学院安徽 合肥 230036 
王萍 安徽农业大学动物科技学院安徽 合肥 230036 
彭秀娟 安徽农业大学动物科技学院安徽 合肥 230036 
刁迎珠 安徽农业大学动物科技学院安徽 合肥 230036 
李文慧 安徽农业大学动物科技学院安徽 合肥 230036 
伍亮 安徽农业大学动物科技学院安徽 合肥 230036 
张志强 安徽农业大学动物科技学院安徽 合肥 230036 
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中文摘要:
      为探究增温对蝌蚪形态表型和生理特征的影响,设置了26℃、28℃和30℃ 3个温度梯度组,每组设平行处理3次,测定了黑斑侧褶蛙(Pelophylax nigromaculatus)从37 期蝌蚪发育至46期幼蛙的时长、以及46期幼蛙身体和内脏器官大小、血液中各型白细胞的百分比及嗜中性粒细胞与淋巴细胞的比值(N/L)、植物血凝素(phytohemagglutinin,PHA-P)反应的变化情况。结果显示:(1)随着温度升高,变态时长缩短,但组间差异不显著(P>0.05);变态完成期幼蛙的体型随温度升高显著变小(P<0.05),肾脏湿重系数下降明显(P<0.05),其他内脏器官湿重系数和消化道各段长度系数的组间差异均不显著(P>0.05)。(2)增温使幼蛙血液中嗜中性粒细胞的百分比和N/L下降(P<0.05),但嗜碱性粒细胞的百分比明显增加(P<0.05)。(3)26℃组、28℃组和30℃组幼蛙PHA-P反应最高值分别出现于注射后1 h、2 h和2 h,组间差异均不显著(P>0.05)。增温使幼蛙的体型趋于变小、肾脏湿重下降,免疫功能和应激反应能力均趋弱,嗜中性粒细胞与嗜碱性粒细胞的数量存在权衡关系,气候变暖将不利于幼蛙的存活。
英文摘要:
      In this study, we explored the effects of increasing temperature on the morphological phenotype and physiological traits on the tadpoles of dark-spotted frogs, Pelophylax nigromaculatus. Three temperature treatments (26oC, 28oC, and 30oC ) were set for the study, with each treatment run in triplicate. The metamorphosis duration from Gosner stage 37 tadpoles to Gosner stage 46 froglets (complete metamorphosis) was recorded, and the morphological phenotypes and physiological traits of Gosner stage 46 froglets were determined, including the sizes of body and internal organs, percentage of different leukocyte subtypes in the blood, ratio of neutrophils to lymphocytes (N/L), and responsiveness to phytohemagglutinin (PHA-P). One-way ANOVA and multiple comparisons were used to analyze differences among the temperature treatment groups. Results show: (1) The increased temperature shortened the metamorphosis duration from Gosner stage 37 tadpoles to stage 46 froglets, but no significant differences were found among the temperature groups (P>0.05);The body size of froglets, as well as the kidney wet mass index decreased significantly with increased temperatures (P<0.05); No significant differences were found among different temperature treatments for any other organ wet mass index or the length index of different digestive tract segments (P>0.05). (2) With increased temperature, both the percentage of neutrophils and ratio of N/L decreased while the percentage of basophils significantly increased (P<0.05). (3) The maximal value for the PHAP-P response of froglets in the 26oC, 28oC, and 30oC treatments appeared at 1 h, 2 h, and 2 h after injection, and the differences were not significant (P>0.05). Increased temperatures shortened the body size of froglets and reduced kidneys wet mass at complete metamorphosis stage, weakened stress response capacity and immune function, and a tradeoff occurred between the number of neutrophils and basophils. We therefore conclude that climate warming is detrimental to the survival of froglets.
裴鑫怡,王萍,彭秀娟,刁迎珠,李文慧,伍亮,张志强.2024.增温对黑斑侧褶蛙蝌蚪形态表型和生理特征的影响[J].水生态学杂志,45(3):165-171.
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