长江流域好氧不产氧光合异养细菌的空间分布及其影响因素
Spatial Distribution and Environmental Factors that Influence Aerobic Anoxygenic Photosynthetic Bacteria in the Yangtze River Basin
投稿时间:2021-06-03  修订日期:2022-11-22
中文关键词:光合异养细菌  空间分布  基因丰度  环境因子  长江流域
英文关键词:photosynthetic heterotrophic bacteria  spatial distribution  gene abundance  environmental factors  Yangtze River basin
基金项目:广西自然科学基金(2022GXNSFAA035572);中国地质科学院基本科研业务费项目(2016001, 2020004);国家自然科学基金(41402238);成都工业学院校级项目(2021ZR023)
作者单位
何若雪 成都工业学院学科建设与科技发展处四川 成都 611730中国地质科学院岩溶地质研究所自然资源部、广西岩溶动力学重点实验室广西 桂林 541004 
于 奭 中国地质科学院岩溶地质研究所自然资源部、广西岩溶动力学重点实验室广西 桂林 541004 
孙平安 中国地质科学院岩溶地质研究所自然资源部、广西岩溶动力学重点实验室广西 桂林 541004 
何师意 成都工业学院学科建设与科技发展处四川 成都 611730 
张春来 中国地质科学院岩溶地质研究所自然资源部、广西岩溶动力学重点实验室广西 桂林 541004 
吴 夏 中国地质科学院岩溶地质研究所自然资源部、广西岩溶动力学重点实验室广西 桂林 541004 
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中文摘要:
      为探讨长江流域水体微生物和光合异养细菌基因丰度的空间分布特征及其对环境因子的响应机制,于2016年10月沿长江流域干流和主要一级支流进行了采样调查。利用分子生态学技术,分析了长江流域干流和主要一级支流水体微生物16S rRNA基因和好氧不产氧光合异养细菌(Aerobic Anoxygenic Phototrophic Bacteria,AAPB)pufM基因丰度的空间分布特征,并讨论了其环境驱动机制。结果表明,流域内16S rRNA基因丰度为0.02×106~811.78×106 copies/L,变幅高达40 589倍;pufM基因在各样品中均有分布,丰度为0.62×102~250.86×102 copies/L,变幅高达405倍,16S rRNA基因和pufM基因丰度均无明显的空间变化规律。研究区16S rRNA和pufM基因丰度与水体浮游植物及藻类活动负相关,表现出明显的竞争关系,但岩溶水库坝前采样点的AAPB相对丰度明显高于其他采样点。相比地表河,岩溶水库环境更有利于AAPB生存,其平缓流速有利于AAPB形成集群或聚合物沉降,岩溶水库沉积较多的有机质为AAPB提供了充足的有机质底物。研究结果填补了长江流域AAPB分布情况的空白,可为后续流域内碳循环的相关生态作用研究提供参考。
英文摘要:
      This study explored the spatial distribution of micro-organisms and the abundance of aerobic anoxygenic phototrophic bacteria (AAPB) in the mainstream and primary tributaries of the Yangtze River basin as well as their response to environmental factors. In October 2016,water and micro-organism samples were collected at 14 sampling sites in the mainstream and primary tributaries of Yangtze River and water quality parameters at each sampling site were monitored simultaneously. Molecular biological techniques were used in this study to determine the abundance of the micro-organism 16S rRNA gene and AAPB pufM gene. The abundance of the 16S rRNA gene ranged from 0.02×106 copies/L to 811.78×106 copies/L (a variation of factor 40 589). The pufM gene ranged from 0.62×102 copies/L to 250.86×102 copies/L (factor 405). Neither gene displayed obvious rules of spatial variation. The 16S rRNA and the pufM genes competed with the phytoplankton and algal communities, based on the negative correlation of gene abundance with environmental factors indicating phytoplankton and algal activity. However, the relative abundance of AAPB at the sampling site above the karst reservoir dam was significantly higher than that at other sampling sites, indicating that the karst environment is more conducive to AAPB survival than typical rivers. The low flow velocity is conducive to AAPB cluster formation and settling, and the organic matter deposited in the reservoir provides sufficient substrate for AAPB growth. The results of this study fill the research gap on AAPB distribution in the Yangtze River basin and provide a basis for subsequent research on the role of AAPB in carbon cycling in the river basin.
何若雪,于 奭,孙平安,何师意,张春来,吴 夏.2022.长江流域好氧不产氧光合异养细菌的空间分布及其影响因素[J].水生态学杂志,43(6):69-77.
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