盐度对大鳞副泥鳅抗氧化酶及组织结构的影响
Effects of Salinity on Antioxidant Enzymes and Histological Structure of Paramisgurnus dabryanus
投稿时间:2021-05-23  修订日期:2022-11-21
中文关键词:大鳞副泥鳅  盐度  Na+-K+-ATP酶  抗氧化酶  组织结构
英文关键词:Paramisgurnus dabryanus  salinity  Na+-K+-ATPase  antioxidant enzymes  histological structure
基金项目:江苏省重点研发计划(现代农业)(BE2018387);江西省现代农业产业技术体系建设专项资金(JXARS-10);农业农村部热带亚热带水产资源利用与养殖重点实验室开放课题(2019PR03)
作者单位
李 雪 中国水产科学研究院珠江水产研究所农业农村部热带亚热带水产资源利用与养殖重点实验室广东 广州 510380江苏海洋大学海洋科学与水产学院江苏省海洋生物技术重点实验室江苏 连云港 222005 
赵 建 中国水产科学研究院珠江水产研究所农业农村部热带亚热带水产资源利用与养殖重点实验室广东 广州 510380 
王玮欣 江苏海洋大学海洋科学与水产学院江苏省海洋生物技术重点实验室江苏 连云港 222005 
朱洪赓 江苏海洋大学海洋科学与水产学院江苏省海洋生物技术重点实验室江苏 连云港 222005 
陈建华 中国水产科学研究院珠江水产研究所农业农村部热带亚热带水产资源利用与养殖重点实验室广东 广州 510380江苏海洋大学海洋科学与水产学院江苏省海洋生物技术重点实验室江苏 连云港 222005 
朱 明 江苏海洋大学海洋科学与水产学院江苏省海洋生物技术重点实验室江苏 连云港 222005 
王海华 江西省水产科学研究所农业部湖泊渔业资源环境科学观测实验站江西 南昌 330039 
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中文摘要:
      为研究盐度对大鳞副泥鳅(Paramisgurnus dabryanus)Na+-K+-ATP酶(NKA)、抗氧化酶活性及组织结构的影响,试验设置4、8、12共3个盐度组和1个淡水对照组,以全长(17.60±0.69) cm,体重(35.51±5.30) g的大鳞副泥鳅进行14 d胁迫试验。结果显示,盐度升高使大鳞副泥鳅的鳃Na+-K+-ATP酶活力上升;7 d时盐度8和12组Na+-K+-ATP酶活性均达到峰值,且显著高于淡水对照组(P<0.05)。肝脏SOD和CAT活性均表现为先上升、后下降的趋势,且分别于胁迫12 h和2 d时达到最大值;3个盐度组的GSH-PX酶活性在6 h和12 h均有所升高,且盐度12组显著高于淡水对照组(P<0.05)。盐度4、8和12组肝脏MDA含量分别在1 d和7 d时达到最大值,且显著高于淡水对照组(P<0.05)。组织切片显示,盐度12组的鳃小片间距变大,泌氯细胞数量增多,上皮层被破坏甚至出现溶解现象;肝细胞空泡化严重,血窦扩张范围增大,并出现细胞轮廓模糊,细胞核偏移、溶解。研究表明,大鳞副泥鳅对水体盐度变化具有一定的调节适应能力,但盐度达到12时会对其鳃和肝组织结构造成一定损伤。
英文摘要:
      Paramisgurnus dabryanus, belonging to Paramisgurnus, Cobitidae, is a common freshwater fish species, commercially valuable and highly nutritious. Recent research has found that P. dabryanus shows a degree of salt tolerance. This study explored the effect of salinity on the activities of Na+-K+-ATPase, antioxidant enzymes, and the histological structure of P. dabryanus, aiming to provide new ideas for the low-salt aquaculture of P. dabryanus in saline alkali waters. P. dabryanus of average total length (17.60±0.69) cm and body mass (35.51±5.30) g were selected for the test. Three salinity groups (4, 8, 12 mg/g) and a control group were set, and each treatment was run in triplicate, with 20 test fish for each trial. The test lasted 14 d under the following conditions: water temperature 20-22℃, pH 7.60-7.90, dissolved oxygen 5.2-6.8 mg/L and ammonia nitrogen 0-0.35 mg/L. During the test, the water of each treatment was changed in half daily with the corresponding salinity. After 6 h, 12 h, 1 d, 2 d, 3 d, 7 d and 14 d of the stress test, two fish were sampled for determination of antioxidant enzymes and observation of histological structure. The mortality rates of the control and 4 mg/g salinity groups were 0%, while the mortality rates of the 8 mg/g and 12 mg/g groups were, respectively, (6.67±2.88)% and (23.33±2.88)%. The activity of Na+-K+-ATPase in gills increased with salinity, and the activities of Na+-K+-ATPase in the 8 mg/g and 12 mg/g groups peaked on day 7 and were significantly higher than those in the control and 4 mg/g groups (P< 0.05). The activities of SOD and CAT increased initially and then decreased, peaking, respectively, at 12 h and 2 d. The activity of GSH-PX in the three salinity groups all increased at 6 h and 12 h, and the activity of GSH-PX in the salinity 12 mg/g group was significantly higher than that in the control group (P<0.05). The contents of MDA in the 4 mg/g and 8 mg/g groups peaked on 1 d and the 12 mg/g group on 7 d, all significantly higher than the control group (P<0.05). Histologically, the space between gill lamellae in the 12 mg/g group increased, and the number of chloride-secreting cells increased. The destruction and even dissolution of the epithelial layer were observed in gill lamellae. Meanwhile, serious vacuolization of hepatocytes, dilatation in sinusoids, blurred cellular outline, nuclear deviation and dissolution were observed in the liver. These results indicated that P. dabryanus have strong ability to adapt to the change of water salinity, but salinity of 12 mg/g would result in some damage to the histological structures of gill and liver.
李 雪,赵 建,王玮欣,朱洪赓,陈建华,朱 明,王海华.2022.盐度对大鳞副泥鳅抗氧化酶及组织结构的影响[J].水生态学杂志,43(6):139-145.
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