| 基于mt COI、Cyt b、D-loop序列的南方多鳞鱚群体遗传结构分析 |
| Genetic Structure of Four Sillago sihama Populations in Southern China Based on Mitochondrial Sequences |
| 投稿时间：2021-05-17 修订日期：2021-07-13 |
| DOI：10.15928/j.1674-3075.202105170149 |
| 中文关键词:多鳞鱚 线粒体DNA 遗传结构 遗传多样性 |
| 英文关键词:Sillago sihama mitochondrial DNA genetic structure genetic diversity |
| 基金项目:广西创新驱动发展专项（桂科AA17204088-2） |
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| 中文摘要: |
| 基于线粒体COI、Cyt b、D-loop基因序列对福建厦门（XM）、广东阳江（YJ）、海南海口（HK）和广西北海（BH）的4个多鳞鱚（Sillago sihama）群体进行遗传结构分析。经PCR扩增和测序获得4个多鳞鱚群体195条线粒体COI + Cyt b + D-loop片段序列。结果表明，单倍型多样性指数（Hd）为0.88266~0.98785，核苷酸多样性指数（π）为0.00098~0.00257，整体Hd与π分别为 0.92784 和 0.00158。遗传分化系数（Fst）和基因流（Nm）分别为0.00075~0.14888和2.85851~333.0833。分子方差分析（AMOVA）结果显示，分组间群体内变异比例分别占93.33%、86.57%和94.49%，主要变异均来自群体内。综上，基于COI、Cyt b和D-loop的分析结果，厦门群体和北海、海口、阳江群体间存在中等程度的遗传分化，可作为两个管理保护单位进行保护。本研究为促进中国南方沿海多鳞鱚资源的可持续利用及遗传多样性保护提供参考依据。 |
| 英文摘要: |
| Sillago sihama, commonly known as the sardine, was an important economic species of coastal fishing communities. To restore S. sihama populations, captive breeding and release has been used since successful artificial breeding was accomplished in 2012. In this study, we analyzed the genetic structure of four S. sihama populations in Xiamen, Fujian (XM); Yangjiang, Guangdong (YJ); Haikou, Hainan (HK) and Beihai, Guangxi (BH), based on mitochondrial control region (COI, Cyt b, and D-loop) gene sequences. In January and June 2019, a total of 195 wild S. sihama specimens were collected from the four study areas. Mitochondrial COI + Cyt b + D-loop sequences from the four S. sihama populations were obtained by PCR amplification and sequencing, with respective lengths of 1 274 bp, 1 141 bp and 362-363 bp. The ranges of the haplotype diversity index (Hd) and nucleotide diversity (π) index of the three gene sequences were, respectively, 0.88266-0.98785 and 0.00098-0.00257, with average values of 0.92784 and 0.00158. The ranges of the genetic differentiation coefficient (Fst) and gene flow (Nm) of the four populations were, respectively, 0.01185-0.14888 and 1.429205-333.0833. The four populations were classified according to their geographic positions, and the analysis of molecular variance (AMOVA) showed that genetic variation originated primarily within the populations, with proportions of variation of 93.33% within the four populations (BH, HK, YJ, XM), 86.57% between the south China sea groups (BH, HK, YJ) and east China sea group (XM), and 94.49% between the groups of the east Qiongzhou Straits (BH, HK) and west Qiongzhou Straits (YJ, XM). In summary, there was a moderate degree of genetic differentiation between the Xiamen population and the Beihai, Haikou, and Yangjiang populations that should be protected as two management units. This study provides a reference for the conservation and rational exploitation of S. sihama germplasm resources in south China coast. |
| 彭敏,肖珊,姚久祥,潘传燕,李旻,高雪梅,冯鹏霏,李满园,曾地刚,朱威霖,杨春玲,蒋伟明,陈秀荔.2023.基于mt COI、Cyt b、D-loop序列的南方多鳞鱚群体遗传结构分析[J].水生态学杂志,44(1):108-116. |
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