华南沿海长鳍篮子鱼不同地理群体的遗传多样性分析
Genetic Diversity Analysis of Different Geographical Populations of Siganus canaliculatus Along the South China Coast
投稿时间:2021-04-28  修订日期:2022-09-09
中文关键词:长鳍篮子鱼  遗传多样性  地理群体  线粒体控制区
英文关键词:Siganus canaliculatus  genetic diversity  geographical population  mitochondrial control region
基金项目:国家海水鱼体系北海综合试验站(CARS-47-z17);广西创新驱动发展专项资金项目(桂科AA17204088-2)
作者单位
彭 敏 广西壮族自治区水产科学研究院广西水产遗传育种与健康养殖重点实验室广西 南宁 530021 
肖 珊 广西壮族自治区水产引育种中心广西 南宁530031 
潘传燕 (1.广西壮族自治区水产科学研究院广西水产遗传育种与健康养殖重点实验室广西 南宁 530021 
冯鹏霏 (1.广西壮族自治区水产科学研究院广西水产遗传育种与健康养殖重点实验室广西 南宁 530021 
李 旻 (1.广西壮族自治区水产科学研究院广西水产遗传育种与健康养殖重点实验室广西 南宁 530021 
曾地刚 (1.广西壮族自治区水产科学研究院广西水产遗传育种与健康养殖重点实验室广西 南宁 530021 
杨春玲 广西壮族自治区水产科学研究院广西水产遗传育种与健康养殖重点实验室广西 南宁 530021 
朱威霖 广西壮族自治区水产科学研究院广西水产遗传育种与健康养殖重点实验室广西 南宁 530021 
蒋伟明 广西壮族自治区水产科学研究院广西水产遗传育种与健康养殖重点实验室广西 南宁 530021 
陈秀荔 广西壮族自治区水产科学研究院广西水产遗传育种与健康养殖重点实验室广西 南宁 530021 
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中文摘要:
      为探明华南沿海长鳍篮子鱼(Siganus canaliculatus)不同地理群体遗传多样性与遗传分化状况,采用线粒体控制区(D-loop)基因序列分析华南沿海的厦门(XM)、汕尾(SW)、阳江(YJ)、海口(HK)、三亚(SY)、北海(BH)、钦州(QZ)、防城港(FC)以及越南清化(TH)共9个地理位置的长鳍篮子鱼群体的遗传多样性及群体遗传结构。结果显示,长鳍篮子鱼群体DNA D-loop 序列共计484条,序列长度约828 bp,包含57个变异位点,92个单倍型;所有群体总的单倍型多样性指数(Hd)为0.80798,核苷酸多样性指数(π)为0.00405;群体内遗传距离为0.00386~0.00532,群体间遗传距离为0.00383~0.00482,群体间分化指数为0.01935~0.00759,各种分组的AMOVA分析均显示遗传变异全部来自群体内;Fu's Fs检验值为显著负值(-25.53678,P<0.01),核苷酸错配分布图呈单峰,吻合度检验数值较小且不显著,提示长鳍篮子鱼可能经历过种群扩张事件,推测扩张时间在1.18万~4.74万年。研究表明,南海北部沿岸及东海南部9个长鳍篮子鱼地理群体间不存在显著的地理结构和谱系结构,可划归一个管理单元进行种质资源保护。
英文摘要:
      Siganus canaliculatus, belonging to Siganus, Siganidae, Acanthuridae and Perciformes, is widely distributed along the south China coast. It is an important capture and culture fish species in China with high nutrient and economic values. In this study, we analyzed the genetic structure and historical dynamics of nine S. canaliculatus populations from the south China coast, including Xiamen, Shanwei, Yangjiang, Haikou, Sanya, Beihai, Qinzhou, Fangcheng and Thanh Hoa. The genetic diversity and genetic differentiation status of the nine S. canaliculatus populations were explored based on the mitochondrial control region (D-loop) gene sequences. The aim was to provide reference for the conservation and rational exploitation of wild S. canaliculatus germplasm resources and to evaluate the effect of captive breeding and release. From April 2018 to August 2019, a total of 484 wild S. canaliculatus samples were collected from the nine areas investigated and muscle tissue from each specimen was sampled for D-loop sequence amplification. The length of the 484 DNA D-loop sequences was about 828 bp, containing 57 mutation sites and 92 haplotypes. The overall haplotype diversity and nucleotide diversity indices were 0.80798 and 0.00405, respectively, indicating high haplotype diversity and low nucleotide diversity. Genetic distances within the populations were 0.00386-0.00532, and genetic distances between the populations were 0.00383-0.00482, and the total differentiation index between the populations was -0.01935-0.00759. AMOVA analysis shows that the genetic variation of the S. canaliculatus populations nearly occurred within the population. The neutral test shows that Fu's Fs test value was a significant negative value (-25.53678, P<0.01), the distribution of nucleotide mismatch showed a single peak, and the coincidence test value was small and insignificant, suggesting that S. canaliculatus might have experienced a population expansion event, estimated to occurr about 11 800 years to 47 400 years ago. In summary, there was no significant differentiation of geographic structure and pedigree structure among the nine S. canaliculatus geographical populations in the south China coast, and they can be classified as a management unit for germplasm resource protection.
彭 敏,肖 珊,潘传燕,冯鹏霏,李 旻,曾地刚,杨春玲,朱威霖,蒋伟明,陈秀荔.2022.华南沿海长鳍篮子鱼不同地理群体的遗传多样性分析[J].水生态学杂志,43(5):127-133.
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