| 硫铁矿人工湿地对微污染水源的水质净化效果 |
| Purification Effect of Constructed Wetland on Micro-polluted Water Sources by Using Pyrite Cinder as Filler Media |
| 投稿时间：2018-12-24 修订日期：2022-01-17 |
| DOI：10.15928/j.1674-3075.201812240333 |
| 中文关键词:人工湿地 微污染 硫铁矿 自养型 同步脱氮除磷 |
| 英文关键词:artificial wetland micro-polluted water source pyrite autotrophic simultaneous removal of nitrogen and phosphorus |
| 基金项目:江苏省水利科技项目(2016047) |
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| 中文摘要: |
| 为进一步提升人工湿地在微污染水源水条件下的同步脱氮除磷能力，采用硫铁矿作为湿地填料设计构建了人工湿地装置，并采用挂膜法对硫铁矿进行硫自养型反硝化细菌表面负载，在此基础上研究硫铁矿人工湿地对水体中污染物的去除规律和去除机理，并通过高通量基因测序技术分析研究硫铁矿表面微生物的群落结构。结果显示，从UASB活性污泥中筛选出的硫自养型反硝化菌活性最高，脱氮效果最好；在微污染水源条件下，硫铁矿潜流人工湿地具有较好的同步脱氮除磷能力，在水力停留时间为60 h条件下，其对水体中的化学需氧量（COD）、氨氮（NH3-N）、硝态氮（NO3-N）、总氮（TN）、总磷（TP）的平均去除率分别达到53.5%、60.9%、67.2%、49.2%、46.3%；矿石表面发现菌群群落达到13门以上，变形菌门（Proteobacteria）为矿石表面最为优势的功能微生物菌群，相对丰度比例占45%左右，硫杆菌属（Thiobacillus）为自养反硝化脱氮的主要功能菌属。研究表明，采用硫铁矿作为湿地填料可显著提高人工湿地的脱氮除磷效果，对微污染水体有较好的水质净化效果，以硫源作为反硝化过程的电子供体可以提高低碳源条件下系统的反硝化效果。 |
| 英文摘要: |
| To further improve the capacity of removing nitrogen and phosphorus in constructed wetland, in this study we explored the purification effects of pyrite cinder as the filler media on the micro-polluted water. A vibrating subsurface flow constructed wetland device was constructed using pyrite cinder as a wetland filler and the surface loading of sulphur autotrophic denitrifying bacteria was carried out by using the membrane method. On the basis of this, nitrogen and phosphorus removal rules and mechanism by pyrite cinder in the subsurface flow constructed wetland was studied, meanwhile, the community structure of microbes on the surface of pyrite was analyzed by high-throughput gene sequencing technology. The test water was collected from the Yanlong River, and during June 5th to August 28 of 2018, water quality of three sampling site in the wetland ( Site1, water pump outlet; Site 2, gravel substrate layer of the subsurface upflow wetland; Site 3, pyrite substrate layer) was monitored. The water was sampled every 7 days for the determination of CODCr, NH3-N, NO3-N, TN and TP, and the microbes on the surface of pyrite cinder were collected for the analysis of microbial community structure. Results show that the sulfur autotrophic denitrifying bacteria screened from UASB activated sludge displayed the highest activity and the best effect of removing nitrogen. Under micro-polluted water source conditions, pyrite cinder of the subsurface flow constructed wetland had good capacity of removing nitrogen and phosphorus simultaneously. After 60 h of hydraulic retention, the average removal rates of CODCr, NH3-N, NO3-N, TN and TP by pyrite cinder in the wetland were, respectively, 53.5%, 60.9%, 67.2%, 49.2% and 46.3%. On the surface of the pyrite cinder, more than 13 microbial phyla were detected. Proteobacteria was the most dominant functional microbial flora, with the relative abundance ratio of about 45%. Thiobacillus was the primary functional genus of autotrophic denitrification. As a result, pyrite used as a filler medis in the subsurface constructed wetland significantly improved the effect of nitrogen and phosphorus removal and had good water purification effect on micro-polluted water. Under low carbon source conditions, sulfur source as the electron donor of the denitrification process improved the denitrification effect . |
| 李冬梅,王万忠,马 艳,仓基俊,仲 跃,杜观超,李峰东.2022.硫铁矿人工湿地对微污染水源的水质净化效果[J].水生态学杂志,43(1):56-62. |
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