| 圆口铜鱼人工繁殖及胚胎发育研究 |
| Artificial Propagation and Embryonic Development of Coreius guichenoti |
| 投稿时间：2018-06-08 修订日期：2019-05-15 |
| DOI：10.15928/j.1674-3075.2019.03.016 |
| 中文关键词:圆口铜鱼 原位驯养 人工催产 胚胎发育 |
| 英文关键词:Coreius guichenoti in situ domestication artificial propagation embryonic development |
| 基金项目:圆口铜鱼人工繁殖技术研究（2）（JGJ/024203）；金沙江中游圆口铜鱼人工驯养繁殖技术研究（JS2010/D5） |
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| 中文摘要: |
| 鉴于金沙江梯级电站开发运行、鱼类栖息地减少、洄游通道受阻、自然繁殖受到影响、早期资源量显著下降、种群生存面临危机的现状，为了保护鱼类种质资源，通过人工增殖放流是补充其资源量的主要补救措施。利用船体网箱原位活水驯养的圆口铜鱼（Coreius guichenoti）亲鱼，开展亲鱼驯养、人工催产和胚胎发育研究。结果表明，圆口铜鱼亲鱼在船体网箱活水驯养条件下，性腺发育成熟，可用于人工催产。2017年6月17~30日，采用二次注射法人工催产3批次，共产卵约12万粒，在水温20.5~21.9 ℃条件下，针距8~10 h，效应时间18~19 h，平均受精率69.3%，出苗约5万尾。圆口铜鱼卵径1.9~2.2 mm，卵膜径6.4~7.2 mm，胚胎发育过程分为受精卵、卵裂期、囊胚期、原肠期、神经胚期、器官形成期和出膜期7个阶段，在水温20.5~22.5 ℃，溶氧7 mg/L以上条件下，历时48~62 h出膜。研究结果为圆口铜鱼人工繁殖技术提供了参考，为其规模化人工增殖放流提供了保障。 |
| 英文摘要: |
| Coreius guichenoti is an economic fish species endemic to the upper Yangtze River. Cascaded hydropower development on the Jinsha River has severely altered the habitat and migration routes of C. guichenoti, resulting in a sharp decline of early life stage populations and threatening wild populations. It is thus necessary to conserve C. guichenoti germplasm resources and artificial propagation and release is the primary means available. In this study, we describe and illustrate the propagation technology and embryonic development of C. guichenoti. Parental fish, collected from the Luzhou section of Yangtze River, were reared in-situ in a cage. Then, from June 17-30 of 2017, 24 females and 36 males were selected for artificial spawning and propagation. The gonads matured during in-situ domestication and spawning was induced three times. Fish were injected twice, at an interval of 8-10 h, and the response time was 18-19 h, at a water temperature of 20.5-21.9℃. A total of 120 000 eggs were obtained, with the relative fecundity of 12.05-13.61eggs/g. Artificial fertilization was carried out by the semi-dry method and the average fertilization rate was 69.3%, resulting in a total of 50 thousand fry. The eggs were pelagic, ovate and steel grey, with an average diameter of 1.9-2.2 mm and swelled to 6.4-7.2 mm. Zygotes were incubated at 20.5-22.5℃ and DO >7 mg/L. Embryonic development required 48-62 h and occurred in seven stages: fertilized egg, cleavage, blastula, gastrula, neurula, organ development, and release of newly hatched larvae from the membrane. This study provides a technical reference for large-scale artificial propagation of C. guichenoti. |
| 董纯,唐会元,陈小娟,万成炎,曹恒源.2019.圆口铜鱼人工繁殖及胚胎发育研究[J].水生态学杂志,40(3):115-119. |
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