| 三氯生对雌性黄河鲤激素效应及其机制的研究 |
| Effect of Triclosan (TCS) Exposure on Sex Hormones of Female Yellow River Carp（Cyprinus carpio）and Effect Mechanism |
| 投稿时间：2017-06-13 修订日期：2019-05-14 |
| DOI：10.15928/j.1674-3075.2019.03.018 |
| 中文关键词:三氯生 黄河鲤 雌二醇 睾酮 促性腺激素释放激素 |
| 英文关键词:Triclosan Yellow River carp estradiol testosterone gonadotropin-releasing hormone |
| 基金项目:NSFC-河南人才培养联合基金(U1504303)；河南省高等学校青年骨干教师培养计划项目（2016GGJS-119） |
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| 中文摘要: |
| 为了探讨三氯生(TCS)对雌性鱼类性激素的影响及其机制。通过半静态水质接触染毒法对雌性黄河鲤进行染毒,设置对照组和三个不同浓度组(0.04 mg/L、0.08 mg/L、0.16 mg/L)的TCS处理组,采用酶联免疫(ELISA)和实时荧光定量PCR(RT-PCR)法检测各组雌性黄河鲤血清睾酮(T)和雌二醇(E2)的分泌水平及其卵巢中促性腺激素释放激素(CGnRH-ii-2)和雌激素受体(Er)的mRNA表达水平。结果显示：各浓度组的T水平无显著性差异(P>0.01),而0.16 mg/L的TCS能使黄河鲤血清中的E2含量及E2/T显著性升高(P<0.05, P<0.01)；TCS各浓度组卵巢中CGnRH-ii-2 mRNA水平显著下降(P<0.01),而0.08和0.16mg/L的TCS处理组Er mRNA水平显著升高(P<0.05,P<0.01)。结果表明：TCS具有潜在雌激素效应,其机制与雌激素受体和促性腺激素释放激素基因表达调控相关。 |
| 英文摘要: |
| riclosan (TCS,2,4,4’-trichloro-2’-hydroxydiphenylether), is a broad spectrum antimicrobial agent widely used in personal care products, household cleaning products and medical supplies. Studies have showed that TCS is not readily biodegraded and can accumulate, move up the food chain to humans and produce a variety of toxic effects. However, the effects of TCS on the reproductive endocrine system in fish are poorly understood. In this study, the effect of TCS exposure on the hormones of femaleYellow River carp (Cyprinus carpio) and the effect mechanism were investigated. C. carpio of body length 7.8-9.9 cm were acclimated for one week in a glass aquarium (58cm×28cm ×36cm). After acclimation, the test fish were randomly assigned to a control group and three TCS treatment groups (0.04 mg/L, 0.08 mg/L, and 0.16 mg/L) for a 42-day semi-static exposure under the following conditions: temperature (18±3）℃; DO, >5.0 mg/L; pH, (7.0±0.2). After the experiment, the levels of testosterone (T) and estradiol (E2）in serum, and mRNA levels of CGnRH-ii-2 and estrogen receptor（Er）in the ovary were determined by enzyme-linked immunosorbent assay (ELISA) and quantitative real-time polymerase chain reaction (qRT-PCR). There was no significant difference in the level of testosterone (T) between the control group and any of the TCS treatment groups, but the estradiol (E2）content and the ratio of estradiol to testosterone (E2/T) in test fish serum markedly increased in the 0.16 mg/L TCS treatment group. The mRNA expression of gonadotropin-releasing hormone ii-2 (CGnRH-ii-2) decreased significantly in all treatment groups compared to the control, while mRNA expression of Er in the 0.08 mg/L and 0.16 mg/L TCS treatment groups increased significantly. Therefore, TCS has a potential estrogenic effect related to the regulation of CGnRH-ii-2 and Er in female Yellow River carp. |
| 王凡,徐瑞杰,王 薇.2019.三氯生对雌性黄河鲤激素效应及其机制的研究[J].水生态学杂志,40(3):126-130. |
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