| 锐钛型纳米二氧化钛对河蚬氧化应激的影响 |
| Oxidative Stress Response of Corbicula fluminea Exposed to Anatase Titanium Dioxide Nanoparticles |
| 投稿时间：2015-03-31 修订日期：2015-12-04 |
| DOI：10.15928/j.1674-3075.2015.05.006 |
| 中文关键词:河蚬 锐钛型纳米二氧化钛 抗氧化酶 氧化应激 |
| 英文关键词:Corbicula fluminea anatase titanium dioxide nanoparticles antioxidant enzymes oxidative stress |
| 基金项目:水体污染控制与治理科技重大专项（2014ZX07203010-4；2012ZX07202-004-02） |
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| 中文摘要: |
| 为评价纳米二氧化钛（TiO2-NPs）对河蚬（Corbicula fluminea）的毒性效应，采用5、50、100 mg/L浓度锐钛型TiO2-NPs对河蚬暴露处理24 h、48 h、96 h和192 h，测定其鳃、消化腺和斧足肌肉组织中钛含量以及谷胱甘肽S转移酶（GST）、超氧化物歧化酶（SOD）的活性。结果表明：（1）在相同暴露时间下，河蚬3种组织的平均钛含量均显著上升并高于空白对照组（P<0.05）；随暴露时间延长，消化腺和斧足肌肉中平均钛含量总体上呈上升趋势，且消化腺中钛含量高于斧足组织，而低、中浓度组鳃组织平均钛含量处于较稳定水平；暴露24～192 h，鳃、消化腺和斧足肌肉中平均钛含量分别为0.73～3.50、2.88～32.37、1.15～3.11 μg/g；（2）在暴露初期，河蚬3种组织的GST活性不同程度上升；随暴露时间延长，鳃和消化腺中GST活性先显著下降、后上升，斧足肌肉中GST活性呈现逐步下降趋势；暴露24～192 h，鳃、消化腺和斧足肌肉中GST活性分别为97.39～371.44、164.92～837.22、63.39～247.75 U/mg；（3）暴露24 h后，3个浓度组河蚬鳃和斧足肌肉中SOD活性均明显上升，低浓度组消化腺SOD活性也显著上升；随暴露时间延长，3种组织中SOD活性均呈现逐渐下降趋势，且SOD活性下降幅度与处理浓度呈正相关；暴露24～192 h，鳃、消化腺和斧足肌肉中SOD活性分别为7.68～27.05、3.63～16.92、4.25～43.08 U/mg。研究表明，3种浓度TiO2-NPs暴露导致钛在河蚬体内显著富集并引起氧化应激反应，其对河蚬具有明显的毒性效应。 |
| 英文摘要: |
| In recent years, the wide use of nano-materials has led to accumulation of these materials in water and soil, bringing potential risk to the environmental and ecological health. Anatase titanium dioxide nanoparticles (TiO2-NPs) migrate between aquatic animals, from species lower on the food chain to species higher on the food chain. While there are few reports on the ecotoxicity of titanium to mollusks, Corbicula fluminea is a mollusk with high nutrient content and economic value in China. It grows in a wide range of fresh water habitats and is an important indicator of environment pollution. In this study, titanium bioaccumulation and oxidative stress response of Corbicula fluminea exposed to TiO2-NPs were investigated. Twelve treatment groups with 20 C. fluminea of each group included three TiO2-NPs exposure concentrations (5mg/L, 50 mg/L and 100 mg/L) and four exposure durations (24 h, 48 h, 96 h and 192 h). After exposure, titanium concentrations and enzyme activities of glutathione S-transferase (GST) and superoxide dismutase (SOD) were measured in gill, digestive gland and foot muscle tissues of C. fluminea. Results are as follows: (1) Average titanium content of gill, digestive gland and foot muscle tissues of C. fluminea exposed to TiO2-NPs were significantly higher than for the control group (P< 0.05). Average titanium content in digestive gland and foot muscle increased with time and was higher in the digestive gland than in the foot muscle. Average titanium content in gills was similar in the low and medium TiO2-NPs concentration groups. After exposure for 24 h to 192 h, the ranges of average titanium content in the gill, digestive gland and foot muscle were, respectively; 0.73-3.50 μg/g, 2.88-32.37 μg/g and 1.15-3.11 μg/g. (2) Initially, GST enzyme activities in the three tissues increased, but at different rates. Over time, GST activities in gill and digestive gland tissues rose again after a significant decline, whereas GST activity in foot muscle tissue gradually decreased. After exposure for 24 h to192 h, the ranges of GST activities in the gill, digestive gland and foot muscle tissues were, respectively; 97.39-371.44 U/mg, 164.92-837.22 U/mg and 63.39-247.75 U/mg. After exposure for 24 h, SOD activities in gill and foot muscle tissues increased markedly in all three TiO2-NPs concentration groups, as did SOD activity in digestive gland tissues for the low TiO2-NPs concentration group. SOD activities in the three tissues all gradually declined over time and the rate of decline was proportional to TiO2-NPs concentration. After exposure for 24-192 h, SOD activities in gills, digestive glands and foot muscles ranged, respectively, from 7.68-27.05 U/mg, 3.63-16.92 U/mg and 4.25-43.08 U/mg. Exposure to TiO2-NPs at all three concentrations leads to significant titanium bioaccumulation and oxidative stress reaction in C. fluminea. |
| 王路平,胡涛,张桂蓉,马徐发,魏开建.2015.锐钛型纳米二氧化钛对河蚬氧化应激的影响[J].水生态学杂志,36(5):36-43. |
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