| 低浓度Cd2+胁迫对青鱼组织SOD活性和MT诱导的影响 |
| Effects of Low Concentration of Cd2+ on Superoxide Dismutase Activities and Matellothionein Content in Tissues of Mylopharyngodon piceus |
| 投稿时间：2013-12-12 修订日期：2014-01-02 |
| DOI： |
| 中文关键词:镉 超氧化物歧化酶 金属硫蛋白 青鱼 |
| 英文关键词:Cd SOD Metallothionein Mylopharyngodon piceus |
| 基金项目:浙江省科技计划项目（2011C23062） |
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| 中文摘要: |
| 采用静水试验方法，研究了Cd2+对青鱼的急性毒性效应以及Cd2+安全浓度胁迫对青鱼鳃丝、肌肉和肝脏的超氧化物歧化酶（SOD）和金属硫蛋白（MT）的影响。结果表明：Cd2+对青鱼的96h LC50为1.434mg/L， Cd2+对青鱼的生态毒性为高毒。在0.143 mg/L（安全质量浓度）Cd2+胁迫下，各组织中SOD活性和MT含量在6h、12h内显著上升，呈现出一定时间—效应关系，以暴露6h时最为显著（P<0.05）；暴露12h后，MT的含量基本上趋于平衡，而SOD活性却是快速下降，表明了12h后该浓度Cd2+污染胁迫对SOD活性的抑制作用。整个试验期间，对照组青鱼各组织中的SOD活性和MT含量大小顺序皆为肝脏>鳃丝>肌肉，同种组织内相对比较稳定；肌肉中的SOD活性要显著低于鳃丝和肝脏，而肝脏中的MT含量要显著高于肌肉和鳃丝。
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| 英文摘要: |
| Acute toxicity of waterborne Cd2+ to Mylopharyngodon piceus and the effects of low concentration of Cd2+ on superoxide dismutase（SOD）activities and matellothionein（MT） content in gill, muscle, and liver of Mylopharyngodon piceus were investigated with static exposure techniques. The results showed that 96h LC50 of Cd2+ to Mylopharyngodon piceus was 1.434 mg/L with high eco-toxicity. SOD activities and MT contents in tissues at safe concentration of 0.143mg/L Cd2+increased significantly within 6 hours and 12 hours ,which showed dramatically time effect relationships and the maximal inducing effect was observed within 6 hours(P<0.05). After 12 hours, MT contents were comparatively stable, indicating inducing mechanism was close to the thresholds. In contrast, the SOD activities decreased rapidly after 12hours, which was caused by the inhibitory effects of Cd2+. The activities of SOD and the contents of MT in tissues showed the same trends: liver>gill>muscle. The activity of SOD in muscle was significantly lower than that in gill and liver, while the MT content in liver was significantly higher than that in muscle and gill.
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| 赵汉取,沈萍萍,施沁璇,韦肖杭,朱俊杰.2014.低浓度Cd2+胁迫对青鱼组织SOD活性和MT诱导的影响[J].水生态学杂志,35(2):90-94. |
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