| 基于卵黄蛋白原基因表达的水丝蚓促熟南美白对虾研究 |
| Feasibility of Ripening Litopenaeus vannmei by Limnodrilus hoffmeisteri Based on Vitellogenin Gene Expression |
| 投稿时间：2013-10-31 修订日期：2013-11-25 |
| DOI： |
| 中文关键词:卵黄蛋白原 基因表达 促熟 南美白对虾 水丝蚓 |
| 英文关键词:Vitellogenin gene expression ripening Litopenaeus vannamei Limnodrilus hoffmeisteri |
| 基金项目:广西自然科学基金项目（2011GXNSFD018020）；广西科学研究与技术开发计划项目（桂科合1140010-17）；现代农业产业技术体系建设专项 (CARS-47） |
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| 中文摘要: |
| 针对传统海产亲虾饵料易感染或携带与对虾共患病毒，给育种和苗种繁育工作带来巨大生物安全隐患的问题，以淡水品种水丝蚓作为替代饵料进行南美白对虾亲虾催熟试验，并以卵黄蛋白原基因的表达水平变化验证饵料的催熟效果。设水丝蚓、水丝蚓组合饵料、商业亲虾饲料及传统沙蚕组合饵料等4个饵料组合，分别投喂南美白对虾后备亲虾，测定繁殖性能相关参数，并通过荧光定量PCR技术对各亲虾肝胰腺和卵巢中VTG的表达水平的变化进行了追踪研究。结果显示，水丝蚓组的性腺增重最高，达1475%，连续产卵率最大，平均为72.08%，与传统沙蚕组合饵料组无显著差异性（P>0.05）。南美白对虾肝胰腺和卵巢组织中均存在VTG mRNA，在肝胰腺和卵巢中均呈先上升后下降的表达模式；VTG mRNA的表达量能够一定程度上反映对虾的繁殖性能，有希望发展成为亲虾繁殖性能选育指标；水丝蚓在促进亲虾性腺发育方面效果较传统沙蚕饵料组合好，在改善亲虾产卵效果方面基本与沙蚕组合相当，可作为沙蚕替代饵料用于南美白对虾的亲虾强化培育和催熟。
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| 英文摘要: |
| Traditional broodstock diets, being susceptible to virus attacks or carrying the virus to parent shrimps, bring a huge bio-security risk to shrimp breeding. This study was carried out to determine the feasibility of Limnodrilus hoffmeisteri as a replacement of traditional bait to accelerate maturity of parent shrimps of Litopenaeus vannamei, which was verified by the dynamics of vitellogenin gene expression in hepatopancreas and ovary of Litopenaeus vannmei. The parent shrimps fed on four different diets(commercial parent shrimps feed, Limnodrilus hoffmeisteri combination bait, Limnodrilus hoffmeisteri and Nereis succinea combination bait), and the effect of each diet on the maturity of parent shrimps was evaluated by testing the gonad weight increase rate, eggs amount, average spawning time and levels of VTG gene expression. The results showed that Limnodrilus hoffmeisteri, as a closely related fresh water species of Nereis succinea, had the best effect on maturation of parent shrimps. The highest gonad weight increase rate of 1475% and the largest consecutive spawning rate with average value of 72.08% were both in groups fed on Limnodrilus hoffmeisteri, with no significant difference from the groups fed on Nereis succinea combination bait. The dynamics of VTG mRNA level were observed in hepatopancreas and ovary of Litopenaeus vannmei with the trend of increasing firstly and then decreasing, which reflected reproductive performance of the parent shrimps and was suggested to be an indicator of reproductive performance for selective breeding. As a result, Limnodrilus hoffmeisteri is a suitable substitute of Nereis succinea based on its effect on the reproductive traits and VTG mRNA expression level in parent shrimps of Litopenaeus vannamei.
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| 彭金霞,陈晓汉,陈秀荔,胡睿宇,蒋伟明 ,蒋小珍,李咏梅,韦嫔媛.2014.基于卵黄蛋白原基因表达的水丝蚓促熟南美白对虾研究[J].水生态学杂志,35(2):74-79. |
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