双台河口潮滩湿地不同植被沉积物脲酶、蛋白酶、磷酸酶活性及其与氮、磷含量关系
Activities of Urease, Protease, Phosphatase and Their Relationships with Contents of Nitrogen and Phosphorus in Sediments of Tidal Flat Wetland Vegetation of Shuangtai Estuary
投稿时间:2013-10-14  修订日期:2013-10-30
DOI:
中文关键词:潮滩湿地  脲酶  蛋白酶  磷酸酶  活性
英文关键词:tidal flat wetland  urease  protease  phosphatase  activity
基金项目:国家自然科学基金(41171389),海洋公益性行业科研专项经费(201305043)和辽宁省高等学校优秀科技人才支持计划(LR2013035)
作者单位E-mail
齐继薇 大连海洋大学 海洋科技与环境学院大连 116023 379134911@qq.com 
刘长发* 大连海洋大学 海洋科技与环境学院大连 116023 近岸海洋环境科学与技术辽宁省高校重点实验室大连 116023 liucf@dlou.edu.cn 
刘远 大连海洋大学 海洋科技与环境学院大连 116023 近岸海洋环境科学与技术辽宁省高校重点实验室大连 116023 liuy8848@dlou.edu.cn 
王艺婷 大连海洋大学 海洋科技与环境学院大连 116023 598078055@qq.com 
魏海峰 大连海洋大学 海洋科技与环境学院大连 116023 近岸海洋环境科学与技术辽宁省高校重点实验室大连 116023 weihaifeng@dlou.edu.cn 
李晋 盘锦市海洋与渔业局水产苗种监督管理处辽宁盘锦124010 ln451@163.com 
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中文摘要:
      土壤中生物胞外酶参与土壤中有机物周转和营养物质循环,其活性是土壤中生物群落代谢需求和有效营养物质的直接表现。以2012年7月、9月和11月对双台河口滨海湿地野外调查获取的数据为基础,研究了生长不同植被的沉积物(土壤)脲酶、蛋白酶、磷酸酶活性及其与氮、磷含量的相关关系。翅碱蓬或芦苇单独生长区脲酶活性7月>9月>11月;翅碱蓬和芦苇混合生长区脲酶活性9月>7月>11月;芦苇、苣荬菜、艾蒿混合生长区脲酶活性9月>11月>7月。翅碱蓬单独生长区、翅碱蓬和芦苇混合生长区以及芦苇、苣荬菜、艾蒿混合生长区蛋白酶活性9月>7月>11月;芦苇单独生长区蛋白酶活性9月>11月>7月。翅碱蓬单独生长区、芦苇单独生长区磷酸酶活性7月>9月>11月;翅碱蓬和芦苇混合生长区磷酸酶活性9月>11月>7月;芦苇、苣荬菜、艾蒿混合生长区磷酸酶活性9月>7月>11月。除少数样点外,不同土层的脲酶、蛋白酶和磷酸酶活性0~10cm土层>10~20cm土层>20~30cm土层。脲酶活性与土壤全氮含量正相关,蛋白酶活性与氨氮含量正相关,磷酸酶活性与速效磷含量正相关。河口滨海潮滩湿地生长不同植被的沉积物(土壤)脲酶、蛋白酶、磷酸酶活性不同,存在季节差异,且与沉积物中不同形态的氮、磷含量存在正相关关系。
英文摘要:
       Soil extracellular enzymes mediate organic matter turnover and nutrient cycling, of which the activities are the direct expression of the soil community to metabolic requirements and available nutrients. Based on the measured data of sediments sampled from tidal flat wetland of Shuangtai estuary in July, September and November 2012, the activities of urease, protease and phosphatase and their relationships with contents of nitrogen and phosphorus in the sediments from different vegetation were analyzed. In terms of temporal characteristics, the activity of urease in the sediments of Suaeda heteroptera or Phragmites communis planted region was in the order of July > September > November, while in the sediments of companion planting of S. heteroptera and P. communis, the order was September > July > November, and the order of urease activity was September > November > July in the sediments of companion planting of S. heteroptera, Sonchus brachyotus and Artemisia argy. In respect to the activity of protease, the order of activity in the sediments of S. heteroptera planted region, in the sediments of companion planting of S. heteroptera and P. communis, and in the sediments of companion planting of P. communis, S. brachyotus and A. argy was all in the order of September > July > November, while the order was September > November > July in the sediments of P. communis planted region. The order of phosphatase activity was July > September > November in the sediments of S. heteroptera or P. communis planted region, while the order was September > November > July in the sediments of companion planting of S. heteroptera and P. communis, and September > July > November in the sediments of companion planting of P. communis, S. brachyotus and A.argyi. Comparing from the vertical layers, the order of activities of urease, protease and phophatase was 0 – 10 cm > 10 – 20 cm > 20 – 30 cm except the few sampling sites. Positive correlation was observed between the urease activity and the content of total nitrogen, as well as the protease activity with the content of ammonia nitrogen, and the phosphatase activity with the content of available phosphorus. The activities of urease, protease and phosphatase in the sediments from different vegetation type of Shuangtai estuary were different with obvious seasonal variations, which were all positively correlated to the contents of different forms of nitrogen and phosphorus in the sediments.
齐继薇,刘长发,刘远,王艺婷,魏海峰,李晋.2014.双台河口潮滩湿地不同植被沉积物脲酶、蛋白酶、磷酸酶活性及其与氮、磷含量关系[J].水生态学杂志,35(4):1-7.
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