| 附着藻类对太湖水中3种氮源的吸收作用 |
| Uptake of Three Different Nitrogen Forms by Periphytic Algae in Taihu Lake, China |
| 投稿时间：2013-06-06 修订日期：2013-11-11 |
| DOI： |
| 中文关键词:附着藻类 氮吸收 尿素 NH4+ NO3- 太湖 |
| 英文关键词:periphytic algae nitrogen uptake urea ammonium nitrate Taihu Lake |
| 基金项目:中国科学院南京地理与湖泊研究所“一三五”战略发展规划项目”（NIGLAS2012135007）; 国家重大科学研究计划项目（2012CB956104） |
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| 中文摘要: |
| 利用15N标记技术，比较了附着藻类对太湖3种氮源（尿素、NH4+和NO3-）的吸收能力。附着藻类对尿素的吸收速率为1.93～5.52μmol/(g.h)，对NH4+的吸收速率为0.89～7.67μmol/(g.h)，而对NO3-的吸收速率为0.09～0.35μmol/(g.h)。各氮源的贫化百分比在0.18 %～9.03 %。在浓度为20和50μmol/L时，尿素的贫化值分别为8.41 %和8.14 %，与NH4+的贫化值相当（分别为9.03 %和 7.01 %）；NO3-的贫化值在各浓度下都低于0.29 %。附着藻类对每种氮源的吸收速率在3种氮源吸收速率总和中的相对比重：尿素平均为47.9 %，NH4+平均为49.6 %，NO3-平均为2.5 %。根据Vmax与Ks比值，附着藻类对3种氮源的吸收亲和力大小顺序为：尿素＞NH4+＞NO3-。研究表明，尿素是除NH4+外附着藻类生长可利用的又一重要氮源。 |
| 英文摘要: |
| The uptake ability of periphytic algae to three different nitrogen substrates (urea, NH4+ and NO3-) was examined using 15N tracer techniques in eutrophic Lake Taihu in China.The periphytic algae uptake rates of urea and NH4+ were 1.93～5.52μmol/(g•h) and 0.89～7.67μmol/(g•h), respectively, accounting for 47.9 % and 49.6 % of the total N uptake rates ,which were significantly higher than that of NO3- (0.09～0.35μmol/(g•h)) accounting for 2.5 % to the total N uptake rates.The depletion rates of each nitrogen source ranged from 0.18 % to 9.03 % in the experiment. At concentrations of 20μmol/L and 50μmol/L, the depletion rates of urea were 8.41 % and 8.14 % respectively , 9.03 % and 7.01 % for NH4+ , while both much lower than 0.29 % for NO3- . According to the Vmax : KS ratio of periphytic algal uptake kinetics, the sequence of uptake affinity was urea＞NH4+＞NO3-. Our results suggested that the ability to absorb urea gives periphytic algae access to additional nitrogen source in eutrophic lakes. |
| 张强,刘正文.2014.附着藻类对太湖水中3种氮源的吸收作用[J].水生态学杂志,35(1):60-64. |
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